RMI2 CRISPR/Cas9 KO Plasmid (h): sc-407104
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RMI2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RMI2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RMI2 CRISPR/Cas9 KO Plasmid (h) | sc-407104 | 20 µg | $397.00 |
Overview
RMI2 (RecQ mediated genome instability 2) encodes a conserved component of the BLM–TOP3A–RMI1/2 dissolvasome that promotes dissolution of double Holliday junctions to suppress sister chromatid exchanges and maintain genome stability. By coordinating homologous recombination intermediate processing, RMI2 supports faithful replication fork restart and limits aberrant crossover events during DNA repair. This activity links RMI2 to core DNA damage response pathways and cell-cycle checkpoint control under replicative stress. Disruption of dissolvasome function, including altered RMI2 activity, is associated with elevated chromosomal instability and phenotypes observed in Bloom syndrome–related genome maintenance defects, making it relevant for studying mechanisms that contribute to cancer-associated mutational processes.
RMI2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RMI2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RMI2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RMI2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RMI2 protein expression.
This CRISPR knockout system enables efficient generation of RMI2-deficient cell models for investigation of RMI2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RMI2 exon(s) critical for RMI2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RMI2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RMI2 CRISPR/Cas9 KO Plasmid (h) and RMI2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RMI2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RMI2 HDR Plasmid (h) and RMI2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RMI2 homology arms to support homology-directed repair at defined RMI2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.