RFX4 CRISPR/Cas9 KO Plasmid (h): sc-406366
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RFX4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RFX4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RFX4 CRISPR/Cas9 KO Plasmid (h) | sc-406366 | 20 µg | $397.00 |
Overview
RFX4 (regulatory factor X 4) is a winged-helix transcription factor in the RFX family that binds X-box–like promoter elements and helps coordinate cell type–specific gene expression programs. In human biology, RFX4 is strongly linked to neurodevelopmental processes, including regulation of transcriptional networks that influence ciliogenesis, ventricular development, and differentiation states in the central nervous system. Altered RFX4 activity has been associated with disrupted brain patterning and neurodevelopmental phenotypes, making it a useful entry point for studying transcription factor–driven regulatory circuitry. Because RFX proteins can intersect with pathways governing chromatin accessibility and cilia-related gene modules, RFX4 perturbation is relevant for mechanistic studies of neuronal lineage control and ciliary signaling contexts.
RFX4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RFX4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RFX4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RFX4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RFX4 protein expression.
This CRISPR knockout system enables efficient generation of RFX4-deficient cell models for investigation of RFX4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RFX4 exon(s) critical for RFX4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RFX4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RFX4 CRISPR/Cas9 KO Plasmid (h) and RFX4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RFX4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RFX4 HDR Plasmid (h) and RFX4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RFX4 homology arms to support homology-directed repair at defined RFX4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.