RbAp46 Antibody (E-9): sc-377197

RbAp46 Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects RbAp46 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-RbAp46 antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RbAp46 plays a crucial role in chromatin remodeling, which is essential for the regulation of gene expression. RbAp46 interacts with histones and serves as a key component of the histone deacetylase complex, influencing the acetylation status of histones. This modification is vital because acetylation of lysine residues in histone tails leads to a more relaxed chromatin structure, allowing transcription factors better access to DNA, thereby promoting gene expression. Conversely, deacetylation of histones is linked to transcriptional repression, highlighting RbAp46′s role in maintaining the balance of gene expression. RbAp46′s ability to modulate chromatin structure underscores its significance in cellular processes such as differentiation, proliferation, and response to external signals, making RbAp46 a valuable target for research in cancer and other diseases where gene regulation is disrupted.

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RbAp46 Antibody (E-9) References:

1. Rb-associated protein 46 (RbAp46) suppresses the tumorigenicity of adenovirus-transformed human embryonic kidney 293 cells.  |  Guan, LS., et al. 2001. Int J Cancer. 93: 333-8. PMID: 11433396
2. Structural basis for the recognition of histone H4 by the histone-chaperone RbAp46.  |  Murzina, NV., et al. 2008. Structure. 16: 1077-85. PMID: 18571423
3. SUMO-1 overexpression increases RbAp46 protein stability and suppresses cell growth.  |  Giri, R., et al. 2008. Anticancer Res. 28: 3749-56. PMID: 19189660
4. Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.  |  Qian, YW. and Lee, EY. 1995. J Biol Chem. 270: 25507-13. PMID: 7503932
5. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
6. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
7. Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
8. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p.  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
9. A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A.  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
10. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
11. Identification of mouse histone deacetylase 1 as a growth factor-inducible gene.  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381
12. Characterization of a human RPD3 ortholog, HDAC3.  |  Emiliani, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2795-800. PMID: 9501169

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RbAp46 Antibody (E-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-RbAp46 Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RbAp46 Antibody (E-9) Specifications

Alternate Product Name: RbAp46

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-29

Epitope Target: RbAp46

Epitope Species: mouse

Product Reactivity/Detection: RbAp46

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for RbAp46 Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred RbAp46/p48 antibody is RbAp46/p48 Antibody (G-8): sc-373873.