Date published: 2025-9-8

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RANKL 항체 (G-1): sc-377079

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데이터 시트
  • RANKL 항체 G-1 는 마우스 monoclonal IgG1 κ RANKL 항체, 44간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 RANKL의 46-317 아미노산을 항원으로 사용하였습니다.
  • RANKL 항체 (G-1)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 RANKL 를 감지하는 데에 추천한다.
  • 항-RANKL 항체(G-1)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • RANKL (G-1): sc-377079무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP는 RANKL 항체(G-1) WB 애플리케이션용입니다. 이 시약은 이제 RANKL 항체(G-1)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

더보기

RANKL 항체(G-1)는 마우스, 쥐 및 인간 유래의 RANKL 단백질을 WB, IP, IF 및 ELISA로 검출하는 IgG1 κ 마우스 단일 클론 RANKL 항체(TNFSF11 항체, TNF 슈퍼 패밀리 멤버 11 항체 또는 CD254 항체로도 지정됨)입니다. RANKL 항체(G-1)는 비접합 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항체로도 제공됩니다. 종양괴사인자(TNF) 수용체 수퍼패밀리의 구성원은 TNF 수용체 관련 인자(TRAF) 계열의 신호 분자와 상호 작용하여 NFκB 및 JNK 경로를 활성화합니다. RANK(수용체 활성화 인자)는 수지상 세포에서 확인되는 TNFR 계열의 일원입니다. 이 제1형 막 수용체는 광범위한 조직에서 발현됩니다. RANK의 C-말단은 RANK가 TRAF2, 5 및 6과 결합하는 데 필요하며, NFκB 활성화를 자극하는 데도 필요합니다. 이 수용체의 리간드인 RANKL(TRANCE, OPGL 또는 ODF라고도 함)은 주로 림프 조직과 T 세포주에서 발현되는 II형 막 통과 단백질입니다. RANKL은 T세포와 파골세포의 중요한 조절자인 것으로 보입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

RANKL 항체 (G-1) 참고문헌:

  1. [골다공증과 RANKL 신호].  |  Nakamura, M. and Udagawa, N. 2011. Clin Calcium. 21: 1149-55. PMID: 21814019
  2. 골다공증 및 암 관련 뼈 질환에 대한 임상 적용에 필요한 RANKL.  |  Yasuda, H. 2013. World J Orthop. 4: 207-17. PMID: 24147256
  3. 면역과 암에 대한 RANKL 표적 치료의 효과.  |  Cheng, ML. and Fong, L. 2014. Front Oncol. 3: 329. PMID: 24432249
  4. 파골세포 전구체에 대한 RANKL 제시의 조절 메커니즘.  |  Honma, M., et al. 2014. Curr Osteoporos Rep. 12: 115-20. PMID: 24477414
  5. 유방암에서 RANK/RANKL 경로의 역할.  |  Kiesel, L. and Kohl, A. 2016. Maturitas. 86: 10-6. PMID: 26921922
  6. SH3BP2 체루비즘 마우스에서의 RANKL 독립적 파골세포 형성.  |  Kittaka, M., et al. 2020. Bone Rep. 12: 100258. PMID: 32258251
  7. 비스포스포네이트 치료 쥐에서 국소 RANKL 전달이 소켓 치유를 개선합니다.  |  Soundia, A., et al. 2021. Bone. 148: 115945. PMID: 33836308
  8. RANKL 억제는 섬유성 이형성증을 가진 마우스에서 병변 진행을 멈추고 골 재광화를 촉진합니다.  |  Liu, Z., et al. 2022. Bone. 156: 116301. PMID: 34952228
  9. [RANKL의 발견과 연구의 현재와 미래 영향].  |  Yasuda, H. 2023. Nihon Yakurigaku Zasshi. 158: 247-252. PMID: 37121707
  10. RANKL 신호는 골격근을 산화적 프로파일로 유도합니다.  |  Cavalcanti de Araújo, PH., et al. 2024. J Bone Miner Res. 39: 753-764. PMID: 38619281

주문정보

제품명카탈로그 번호 단위가격수량관심품목

RANKL 항체 (G-1)

sc-377079
200 µg/ml
$316.00

RANKL (G-1): m-IgG Fc BP-HRP 번들

sc-529968
200 µg Ab; 10 µg BP
$354.00

RANKL (G-1): m-IgGκ BP-HRP 번들

sc-523176
200 µg Ab, 40 µg BP
$354.00

RANKL (G-1): m-IgG1 BP-HRP 번들

sc-543794
200 µg Ab; 20 µg BP
$354.00

RANKL 항체 (G-1) AC

sc-377079 AC
500 µg/ml, 25% agarose
$416.00

RANKL 항체 (G-1) HRP

sc-377079 HRP
200 µg/ml
$316.00

RANKL 항체 (G-1) FITC

sc-377079 FITC
200 µg/ml
$330.00

RANKL 항체 (G-1) PE

sc-377079 PE
200 µg/ml
$343.00

RANKL 항체 (G-1) Alexa Fluor® 488

sc-377079 AF488
200 µg/ml
$357.00

RANKL 항체 (G-1) Alexa Fluor® 546

sc-377079 AF546
200 µg/ml
$357.00

RANKL 항체 (G-1) Alexa Fluor® 594

sc-377079 AF594
200 µg/ml
$357.00

RANKL 항체 (G-1) Alexa Fluor® 647

sc-377079 AF647
200 µg/ml
$357.00

RANKL 항체 (G-1) Alexa Fluor® 680

sc-377079 AF680
200 µg/ml
$357.00

RANKL 항체 (G-1) Alexa Fluor® 790

sc-377079 AF790
200 µg/ml
$357.00

Hello! I purshed conjugate antibody aganist RANKL 377079HRP in order to detect RANKL in a bone sample by IHC(P) and I followed the immunoperoxidase staining protocol that you provide on-line. However, the processing is not working. Can you help me?

Asked by: Valesca
Thank you for your question. Please reach out to your local distributor directly for assistance troubleshooting your IHC results. They will be happy to help troubleshoot and discuss your results with you, and will work with Santa Cruz as needed.
Answered by: Tech Service
Date published: 2019-08-06

Does it work well on primary cell lysates?

Asked by: flowersf
Thank you for your question. RANKL Antibody (G-1): sc-377079 is recommended for detection of RANKL of mouse, rat and human origin by WB, IP, IF and ELISA. This recommendation is valid for lysates generated from both cell cultures and tissue material.
Answered by: Tech Support Europe
Date published: 2019-07-09

Boa Tarde! Nos comentários vocês não indicam IHQ para bloco parafinado, porém nas especificações conta isso "disponível conjugado a agarose para IP; HRP para WB, IHC (P) e ELISA; e para ou ficoeritrina ou FITC para IF, IHC (P) e FCM" Estou com duvidas!

Asked by: Janaína
Thank you for your question. The conjugated form of this antibody is recommended for IHC on paraffin sections, however, the non-conjugated form has been warrantied for IF on fresh frozen tissue sections.
Answered by: Tech Service
Date published: 2019-06-20

Which is the actual molecular weight of RANKL? the data sheet says 40-35 Kd, but I have got bands around 50Kd when using primary clavaria cells from mice...

Asked by: MP03
Thank you for your question. The expected molecular weight of RANKL, as detected by RANKL Antibody (G-1): sc-377079, depends on the form present in your sample: RANKL full-length (35-40 kDa), membrane-bound RANKL (34-40 kDa), or soluble RANKL (20-30 kDa) . If you are detecting a signal at a different height, please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

What's the difference between the sc-52950 and the sc-377079 HRP in IHC with mouse tissue? And what about the result?Will they be same? 

Asked by: sumer
Thank you for your inquiry. Both sc-52950 and sc-377079 are mouse monoclonal antibodies recommended for detection of RANKL of mouse, rat and human origin. sc-52950 can be used on paraffin-embedded tissues, while sc-377079 is not recommended for such application. sc-377079 HRP is sc-377079 directly conjugated to HRP. When using sc-377079 HRP in WB or IHC, the secondary reagent can be avoided. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. Best of luck with your research!
Answered by: Technical Support 14
Date published: 2017-07-10

How much protein do you recommend loading on the gel for Western blot detection using RANKL (G-1): sc-377079 monoclonal antibody?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 5 out of 5 by from Ottimo funzionamentoL'anticorpo funziona bene per immunoprecipitazione
Date published: 2024-10-17
Rated 3 out of 5 by from RANKL - Dot BlotRANKL Antibody works also for Dot Blot. The Antibody have been diluted 1:1000 in TBS-T buffer.
Date published: 2019-11-04
Rated 3 out of 5 by from RAW 264.7Hard to work in the WB of osteoclast induced from RAW 264.7,with 1:500.
Date published: 2017-10-23
Rated 4 out of 5 by from Good bandsThis antibody works fine on western blot in 1:500. The bands are clear.
Date published: 2017-08-25
Rated 5 out of 5 by from good performanceI used it for IF on HeLa cells (1:300 dilution). It works both on PFA and methanol-fixed cells.
Date published: 2017-05-28
Rated 5 out of 5 by from specific bandUsed this antibody to make WB in 1:600 dilution, we got obtained specific band.
Date published: 2017-05-27
Rated 5 out of 5 by from strong bands.Strong bands detected in WB for human lung tissue, use dilution 1:400.Recommend use it.
Date published: 2017-02-09
Rated 5 out of 5 by from recombinant RANKLIt works great in WB, favorable band achieved in mouse recombinant RANKL.
Date published: 2017-02-07
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RANKL 항체 (G-1) is rated 4.3 out of 5 by 15.
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