Rab 1B 항체 (LD-S3) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Rab 1B을 검출할것을 권장합니다.

Rab 1B 항체 (LD-S3): sc-130474

Rab 1B 항체 (LD-S3) is rated 2.5 out of 5 by 2.
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Datasheets

    • 안티-Rab 1B 항체 LD-S3 는 마우스 monoclonal IgG2a (kappa light chain) Rab 1B 항체 이며 100 µg/ml으로 제공합니다.
    • human origin의 Rab 1B의 전체인 91-202 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Rab 1B을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).

Can sc-130474: Rab 1B (LD-S3) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, sc-130474: Rab 1B (LD-S3) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from Not a suitable replacement for sc-599 Unlike the discontinued sc-599 (rabbit anti-Rab1b), which works great on J774A.1 cells, sc-130474 (mouse anti-Rab1b) doesn't work on J774A.1 cells (only produces very dim diffuse staining). Even tried new secondary antibody to make sure that bad secondary antibody was not the problem.
Date published: 2017-05-11
Rated 4 out of 5 by from Signal via western blot analysis of Rab 1B Signal via western blot analysis of Rab 1B expression in A-431 whole cell lysate. -SCBT QC
Date published: 2014-12-05
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