R-Spondin2 CRISPR/Cas9 KO Plasmid (m): sc-433683
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- R-Spondin2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the R-Spondin2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
R-Spondin2 CRISPR/Cas9 KO Plasmid (m) | sc-433683 | 20 µg | $397.00 |
Overview
Rspo2 encodes R-Spondin2, a secreted matricellular ligand that potentiates canonical WNT/β-catenin signaling by engaging LGR receptors and neutralizing RNF43/ZNRF3-mediated turnover of Frizzled receptors. In mouse tissues, R-Spondin2 contributes to developmental patterning, stem/progenitor cell maintenance, and epithelial–mesenchymal signaling, influencing processes such as organogenesis, tissue remodeling, and regeneration. Its activity intersects with pathways controlling proliferation and differentiation, including WNT-driven transcriptional programs and extracellular matrix–dependent morphogen gradients. Dysregulated RSPO2–WNT signaling is frequently studied in contexts of aberrant tissue growth and differentiation, including models of developmental defects and oncogenic pathway activation.
R-Spondin2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rspo2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rspo2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rspo2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish R-Spondin2 protein expression.
This CRISPR knockout system enables efficient generation of Rspo2-deficient cell models for investigation of R-Spondin2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rspo2 exon(s) critical for R-Spondin2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rspo2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by R-Spondin2 CRISPR/Cas9 KO Plasmid (m) and R-Spondin2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rspo2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by R-Spondin2 HDR Plasmid (m) and R-Spondin2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rspo2 homology arms to support homology-directed repair at defined Rspo2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.