PLC beta 1 항체 (D-8) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 PLC β1을 검출할것을 권장합니다.

PLC β1 항체 (D-8): sc-5291

PLC β1 항체 (D-8) is rated 4.5 out of 5 by 10.
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데이터 시트

    • 안티-PLC beta 1 항체 D-8 는 마우스 monoclonal IgG2b (kappa light chain) PLC β1 항체 이며 200 µg/ml으로 제공합니다.
    • rat origin의 PLC β1에서 내부에 위치한 831-1063 아미노산을 항원으로 사용하였습니다.
    • 안티-PLC beta 1 항체 (D-8)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 PLC β1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-PLC beta 1 항체 (D-8)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • PLC β1 (D-8): sc-5291무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 1 out of 5 by from Didn't detect it Used PLCb1 at a 1:200 dilution for WB on NuPAGE gel using HEK293T WCE. Unfortunately, we weren't able to detect anything.
Date published: 2017-09-29
Rated 4 out of 5 by from o/e PLCb1 is detected Overexpressed GFP-PLCb1a in 293 cells was detected. there are no other ns bands. PLCd1 and d3 were not detected. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-07-04
Rated 5 out of 5 by from Well characterized PLC beta 1 ab for WB, IHC & IF Produced more than a dozen citations in the primary literature for WB, IF and IHC(P) on mouse, rat and human samples.
Date published: 2017-01-11
Rated 5 out of 5 by from We have lovely results using this antibody We have lovely results using this antibody.
Date published: 2015-10-09
Rated 5 out of 5 by from Clean Clean, crisp band seen in WB in U-937 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Published data for IF (pfa) in rat hippocampal Published data for IF (pfa) in rat hippocampal neurons and PC12 cells. -SCBT Publication Review
Date published: 2015-04-07
Rated 5 out of 5 by from Published data for WB in mouse friend erythroleukemia Published data for WB in mouse friend erythroleukemia cells. -SCBT Publication Review
Date published: 2015-04-02
Rated 5 out of 5 by from Published data for IF in rat cortex sample Published data for IF in rat cortex sample. -SCBT Publication Review
Date published: 2015-03-25
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