Placental lactogen II Double Nickase Plasmid (h): sc-402301-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Placental lactogen II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Placental lactogen II Double Nickase Plasmid (h) and Placental lactogen II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSH2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Placental lactogen II Double Nickase Plasmid (h) | sc-402301-NIC | 20 µg | $410.00 | |||
Placental lactogen II Double Nickase Plasmid (h2) | sc-402301-NIC-2 | 20 µg | $410.00 |
CSH2 encodes placental lactogen II, a member of the somatotropin/prolactin hormone family produced predominantly by placental trophoblasts. Placental lactogen signaling engages prolactin and growth hormone receptor–related pathways, influencing JAK–STAT and downstream transcriptional programs that regulate maternal metabolic adaptation, fetal growth support, and placental endocrine function. Expression of CSH2 is tightly linked to trophoblast differentiation and placental development, making it a useful marker for studying placental maturation and hormone output. Altered placental lactogen expression has been investigated in the context of placental insufficiency and pregnancy-associated metabolic disturbances, supporting research into endocrine dysregulation at the maternal–fetal interface.
Placental lactogen II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSH2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSH2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSH2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSH2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.