PLAC1 Double Nickase Plasmid (m): sc-425008-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLAC1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PLAC1 Double Nickase Plasmid (m) and PLAC1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Plac1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLAC1 Antibody (G-1): sc-365919
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLAC1 Double Nickase Plasmid (m) | sc-425008-NIC | 20 µg | $410.00 |
Plac1 encodes PLAC1, a placenta-enriched membrane-associated protein implicated in trophoblast differentiation, placental morphogenesis, and regulation of fetomaternal interface biology in mouse. PLAC1 expression is linked to processes such as cell adhesion, migration, and epithelial-like barrier formation that support normal placental development and nutrient exchange. Dysregulated PLAC1 has been associated with aberrant placental growth and reproductive phenotypes, making Plac1 a useful locus for dissecting gene networks that govern extraembryonic development and endocrine signaling. In addition, PLAC1 is frequently studied as an oncofetal antigen in cancer biology, supporting mechanistic research into developmental reprogramming and tumor-associated gene expression programs without implying clinical utility.
PLAC1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Plac1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Plac1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Plac1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Plac1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.