PHF24 CRISPR/Cas9 KO Plasmid (h): sc-409845
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHF24 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHF24 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHF24 CRISPR/Cas9 KO Plasmid (h) | sc-409845 | 20 µg | $397.00 |
Overview
PHF24 (PHD finger protein 24) is a nuclear protein that contains a PHD-type zinc finger, a motif frequently involved in recognizing histone modifications and coordinating chromatin-dependent regulation of transcription. By linking epigenetic state to gene expression programs, PHF24 is positioned to influence neuronal differentiation, synaptic signaling, and activity-dependent transcriptional responses that shape cellular identity. Altered regulation of chromatin readers and transcriptional control networks has been implicated in neurodevelopmental and neuropsychiatric phenotypes, making PHF24 relevant for mechanistic studies of brain-associated pathways. Dissecting PHF24 function can help clarify how epigenetic interpretation interfaces with transcriptional circuitry and downstream cellular phenotypes.
PHF24 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHF24 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHF24 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHF24 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHF24 protein expression.
This CRISPR knockout system enables efficient generation of PHF24-deficient cell models for investigation of PHF24 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHF24 exon(s) critical for PHF24 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHF24 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHF24 CRISPR/Cas9 KO Plasmid (h) and PHF24 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHF24 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHF24 HDR Plasmid (h) and PHF24 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHF24 homology arms to support homology-directed repair at defined PHF24 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.