PDLIM5 CRISPR/Cas9 KO Plasmid (h): sc-405340
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDLIM5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PDLIM5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDLIM5 Antibody (G-2): sc-515621
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDLIM5 CRISPR/Cas9 KO Plasmid (h) | sc-405340 | 20 µg | $397.00 |
Overview
PDLIM5 encodes a PDZ-LIM domain scaffold protein that localizes to actin-rich structures and links cytoskeletal architecture to signaling complexes at focal adhesions and along stress fibers. Through interactions with kinases and actin-associated partners, PDLIM5 contributes to mechanotransduction, cell adhesion and migration, and organization of contractile elements that influence cellular morphology and force transmission. In excitable tissues, PDLIM5 has been connected to regulation of neuronal and cardiac cytoskeletal signaling and synaptic or contractile remodeling. Altered PDLIM5 expression or genetic variation has been reported in studies of neuropsychiatric phenotypes and cardiac pathology, supporting its relevance for investigating cytoskeleton-coupled signaling networks in disease-relevant models.
PDLIM5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PDLIM5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PDLIM5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PDLIM5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PDLIM5 protein expression.
This CRISPR knockout system enables efficient generation of PDLIM5-deficient cell models for investigation of PDLIM5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PDLIM5 exon(s) critical for PDLIM5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PDLIM5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PDLIM5 CRISPR/Cas9 KO Plasmid (h) and PDLIM5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PDLIM5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PDLIM5 HDR Plasmid (h) and PDLIM5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PDLIM5 homology arms to support homology-directed repair at defined PDLIM5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.