Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PARD3B CRISPR/Cas9 KO Plasmid (h): sc-406097
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARD3B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PARD3B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PARD3B Antibody (E-9): sc-398761
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARD3B CRISPR/Cas9 KO Plasmid (h) | sc-406097 | 20 µg | $397.00 |
Overview
PARD3B (PAR-3 family cell polarity regulator beta) is a scaffolding protein that contributes to the establishment and maintenance of apical–basal polarity in epithelial cells by organizing polarity complexes at tight junctions. It functions within conserved polarity networks involving PAR proteins and small GTPase signaling to coordinate asymmetric protein localization, junctional integrity, and cytoskeletal organization. Through these processes, PARD3B influences cell migration, tissue architecture, and signaling compartmentalization that can modulate proliferation and differentiation programs. Dysregulation of polarity regulators, including PARD3B-associated pathways, is frequently studied in the context of epithelial disorganization, invasion-related phenotypes, and other disease-relevant cellular states.
PARD3B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PARD3B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PARD3B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PARD3B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PARD3B protein expression.
This CRISPR knockout system enables efficient generation of PARD3B-deficient cell models for investigation of PARD3B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PARD3B exon(s) critical for PARD3B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PARD3B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PARD3B CRISPR/Cas9 KO Plasmid (h) and PARD3B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PARD3B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PARD3B HDR Plasmid (h) and PARD3B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PARD3B homology arms to support homology-directed repair at defined PARD3B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.