P2Y2 CRISPR/Cas9 KO Plasmid (m): sc-422096
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P2Y2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: P2Y2 Antibody (H-5): sc-518121
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y2 CRISPR/Cas9 KO Plasmid (m) | sc-422096 | 20 µg | $397.00 |
Overview
P2ry2 encodes the mouse P2Y2 receptor, a G protein–coupled purinergic receptor activated by extracellular ATP and UTP. P2Y2 signaling primarily couples to Gq/11 to stimulate phospholipase C, inositol phosphate production, intracellular Ca²⁺ mobilization, and downstream MAPK and PI3K/AKT pathway engagement, shaping transcriptional and cytoskeletal responses. Through regulation of ion transport, chemotaxis, and secretion, P2Y2 contributes to epithelial barrier physiology, endothelial responses, and innate immune signaling in inflammatory microenvironments. Dysregulated purinergic signaling involving P2Y2 has been associated with airway and intestinal inflammation, fibrosis-related remodeling, and tumor-associated stromal and immune interactions, supporting its relevance in disease mechanism studies.
P2Y2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the P2ry2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P2ry2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P2ry2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P2Y2 protein expression.
This CRISPR knockout system enables efficient generation of P2ry2-deficient cell models for investigation of P2Y2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P2ry2 exon(s) critical for P2Y2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P2ry2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P2Y2 CRISPR/Cas9 KO Plasmid (m) and P2Y2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the P2ry2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P2Y2 HDR Plasmid (m) and P2Y2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by P2ry2 homology arms to support homology-directed repair at defined P2ry2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.