p-Atm Antibody (10H11.E12): sc-47739
- p-Atm Antibody (10H11.E12) is a mouse monoclonal IgG1 κ, cited in 126 publications, provided at 200 µg/ml
- raised against a synthetic phosphopeptide corresponding to amino acids Ser 1981 surrounding the Ser 1981 phosphorylation site of human ATM
- Anti-p-Atm Antibody (10H11.E12) is recommended for detection of Ser 1981 phosphorylated Atm of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-p-Atm Antibody (10H11.E12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Atm Antibody (10H11.E12) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Atm Antibody (10H11.E12) (see ordering information below).
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p-Atm Antibody (10H11.E12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 1981 phosphorylated Atm of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p-Atm (10H11.E12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Atm protein plays a crucial role in cellular response to DNA damage, particularly in detecting and repairing double-strand breaks. This function maintains genomic stability, as mutations in Atm gene are associated with ataxia telangiectasia (AT), leading to severe neurological and immunological defects. In response to DNA damage, Atm rapidly activates through autophosphorylation at serine 1981, initiating a cascade of signaling events that lead to cell cycle arrest and DNA repair. Most human tissues predominantly express non-phosphorylated, inactive Atm, highlighting the importance of post-translational modifications in regulating activity and function. anti-Atm antibody (10H11.E12) specifically recognizes phosphorylated Atm, making p-Atm (10H11.E12) monoclonal antibody an invaluable tool for studying DNA damage response mechanisms and pathophysiology of related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-Atm Antibody (10H11.E12) References:
- ATM signaling and genomic stability in response to DNA damage. | Lavin, MF., et al. 2005. Mutat Res. 569: 123-32. PMID: 15603757
- Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM. | Hickson, I., et al. 2004. Cancer Res. 64: 9152-9. PMID: 15604286
- Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma. | Liu, A., et al. 2005. Lab Invest. 85: 436-46. PMID: 15696190
- Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage. | Chen, L., et al. 2005. Cancer Sci. 96: 134-41. PMID: 15723659
- ATM activation in normal human tissues and testicular cancer. | Bartkova, J., et al. 2005. Cell Cycle. 4: 838-45. PMID: 15846060
- Reduced expression of phosphorylated ataxia-telangiectasia mutated gene is related to poor prognosis and gemcitabine chemoresistance in pancreatic cancer. | Xun, J., et al. 2023. BMC Cancer. 23: 835. PMID: 37674118
- PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints. | Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
- DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. | Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
- Discovery of AT gene sparks biomedical research bonanza. | Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
- A single ataxia telangiectasia gene with a product similar to PI-3 kinase. | Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Atm Antibody (10H11.E12) | sc-47739 | 200 µg/ml | $322.00 | |||
p-Atm Antibody (10H11.E12): m-IgG Fc BP-HRP Bundle | sc-528513 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-Atm Antibody (10H11.E12): m-IgGκ BP-HRP Bundle | sc-520908 | 200 µg Ab, 40 µg BP | $361.00 | |||
p-Atm Antibody (10H11.E12): m-IgG1 BP-HRP Bundle | sc-542970 | 200 µg Ab; 20 µg BP | $361.00 | |||
p-Atm Antibody (10H11.E12) AC | sc-47739 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-Atm Antibody (10H11.E12) HRP | sc-47739 HRP | 200 µg/ml | $322.00 | |||
p-Atm Antibody (10H11.E12) FITC | sc-47739 FITC | 200 µg/ml | $336.00 | |||
p-Atm Antibody (10H11.E12) PE | sc-47739 PE | 200 µg/ml | $349.00 | |||
p-Atm Antibody (10H11.E12) Alexa Fluor® 488 | sc-47739 AF488 | 200 µg/ml | $364.00 | |||
p-Atm Antibody (10H11.E12) Alexa Fluor® 546 | sc-47739 AF546 | 200 µg/ml | $364.00 | |||
p-Atm Antibody (10H11.E12) Alexa Fluor® 594 | sc-47739 AF594 | 200 µg/ml | $364.00 | |||
p-Atm Antibody (10H11.E12) Alexa Fluor® 647 | sc-47739 AF647 | 200 µg/ml | $364.00 | |||
p-Atm Antibody (10H11.E12) Alexa Fluor® 680 | sc-47739 AF680 | 200 µg/ml | $364.00 | |||
p-Atm Antibody (10H11.E12) Alexa Fluor® 790 | sc-47739 AF790 | 200 µg/ml | $364.00 |