Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OST48 Double Nickase Plasmid (h): sc-403323-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OST48 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OST48 Double Nickase Plasmid (h) and OST48 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDOST. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OST48 Antibody (E-9): sc-74408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OST48 Double Nickase Plasmid (h) | sc-403323-NIC | 20 µg | $410.00 | |||
OST48 Double Nickase Plasmid (h2) | sc-403323-NIC-2 | 20 µg | $410.00 |
Human DDOST encodes OST48, a core subunit of the oligosaccharyltransferase (OST) complex in the rough endoplasmic reticulum that catalyzes co-translational N-linked glycosylation of nascent secretory and membrane proteins. By supporting glycoprotein folding and quality control, OST48 contributes to ER proteostasis, unfolded protein response signaling, and trafficking through the secretory pathway. Altered N-glycosylation and ER stress are implicated in tumor biology, metabolic dysfunction, and neurodegenerative processes, making DDOST a relevant node for studying glycan-dependent regulation of receptors, transporters, and immune modulators. DDOST/OST48 is also linked to the organization of OST subcomplexes and substrate selectivity, connecting it to broad proteome-wide effects on protein maturation.
OST48 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDOST locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDOST. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDOST function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDOST-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.