Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OSCP Double Nickase Plasmid (h): sc-404116-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OSCP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OSCP Double Nickase Plasmid (h) and OSCP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP5O. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OSCP Antibody (A-8): sc-365162
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OSCP Double Nickase Plasmid (h) | sc-404116-NIC | 20 µg | $410.00 | |||
OSCP Double Nickase Plasmid (h2) | sc-404116-NIC-2 | 20 µg | $410.00 |
ATP5O encodes the oligomycin sensitivity conferral protein (OSCP), a peripheral stalk subunit of mitochondrial ATP synthase (Complex V) that stabilizes the F1Fo holoenzyme and couples proton-motive force to ATP production. OSCP helps maintain efficient oxidative phosphorylation, supporting cellular bioenergetics, mitochondrial membrane potential, and metabolic homeostasis. Perturbation of ATP5O/OSCP can alter electron transport chain function, elevate reactive oxygen species, and trigger compensatory metabolic rewiring. Dysregulated mitochondrial ATP synthase activity and complex V assembly have been implicated in neurometabolic dysfunction and in cancer cell metabolism, making ATP5O a useful target for studying mitochondrial stress signaling.
OSCP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP5O locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP5O. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP5O function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP5O-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.