Olfr291 CRISPR Activation Plasmid (m): sc-434560-ACT

Olfr291 encodes an olfactory receptor in mouse that belongs to the class A G protein–coupled receptor (GPCR) superfamily and is typically associated with sensory neurons in the olfactory epithelium. Upon ligand engagement, olfactory receptors couple to G protein signaling that stimulates adenylyl cyclase, elevates cAMP, and modulates cyclic nucleotide–gated ion channel activity to influence neuronal excitability and odor-evoked responses. Beyond sensory biology, ectopic expression of olfactory receptors has been reported for subsets of receptors in diverse tissues, supporting investigation of GPCR-linked signal transduction, transcriptional programs, and cell-state regulation in noncanonical contexts. Olfr291 is therefore relevant for studies of chemosensory coding, GPCR pathway dynamics, and mechanisms by which receptor expression changes may associate with altered cellular phenotypes in disease models.

Olfr291 CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Olfr291 expression without altering the underlying DNA sequence.

Olfr291 CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Olfr291 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.

Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Olfr291 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Olfr291 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Olfr291 locus and enabling the study of Olfr291-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Olfr291 pathway restoration in tumor cells with silenced or reduced Olfr291 expression.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.