OC-3 CRISPR/Cas9 KO Plasmid (m): sc-434195
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OC-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OC-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OC-3 CRISPR/Cas9 KO Plasmid (m) | sc-434195 | 20 µg | $397.00 |
Overview
Onecut3 (OC-3) is a mouse ONECUT family homeobox transcription factor that binds specific DNA motifs to coordinate cell fate decisions and tissue patterning during development. It contributes to gene regulatory programs involved in differentiation, morphogenesis, and maintenance of specialized cellular identities, integrating with broader transcriptional networks that shape organogenesis. As a nuclear regulator, OC-3 influences downstream pathways governing lineage specification and maturation, making it relevant to studies of developmental biology and epigenetic control of transcription. Dysregulated Onecut3-associated transcriptional programs have been linked in the literature to altered differentiation states and disease-relevant phenotypes in model systems where developmental pathways are perturbed.
OC-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Onecut3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Onecut3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Onecut3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OC-3 protein expression.
This CRISPR knockout system enables efficient generation of Onecut3-deficient cell models for investigation of OC-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Onecut3 exon(s) critical for OC-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Onecut3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OC-3 CRISPR/Cas9 KO Plasmid (m) and OC-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Onecut3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OC-3 HDR Plasmid (m) and OC-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Onecut3 homology arms to support homology-directed repair at defined Onecut3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.