Nucling Double Nickase Plasmid (h): sc-406671-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nucling Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nucling Double Nickase Plasmid (h) and Nucling Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UACA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nucling Antibody (A-5): sc-515005
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nucling Double Nickase Plasmid (h) | sc-406671-NIC | 20 µg | $410.00 | |||
Nucling Double Nickase Plasmid (h2) | sc-406671-NIC-2 | 20 µg | $410.00 |
UACA encodes Nucling, a nuclear protein implicated in the regulation of apoptotic signaling and cellular stress responses, with reported roles in coordinating nuclear events that influence cell survival decisions. Nucling has been linked to modulation of NF-κB-related transcriptional programs and other signal transduction pathways that connect inflammatory cues to apoptosis. Altered UACA expression has been observed in multiple disease-relevant contexts, including cancer and immune-associated conditions, where dysregulated cell death and stress signaling contribute to pathogenesis. As a result, UACA/Nucling is frequently studied in mechanisms of stress-induced apoptosis, transcriptional control, and pathway crosstalk affecting cellular homeostasis.
Nucling Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UACA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UACA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UACA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UACA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.