Date published: 2025-9-7

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Notch1 항체 (A-8): sc-376403

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데이터 시트
  • Notch1 항체 A-8 는 마우스 monoclonal IgM κ Notch1 항체, 50간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 Notch 1에서 C-terminus에 위치한 2425-2444의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Notch 1을 검출할것을 권장합니다.
  • IP에 사용가능한 agarose결합제품; HRP/b> for WB, IHC(P) and ELISA; IF, IHC(P) 와 FCM에 사용가능한 phycoerythrin/b>결합제품, FITC결합제품, Alexa Fluor® 488/b>결합제품 와 Alexa Fluor® 647/b> 결합제품 이 있습니다.
  • Notch1 (A-8): sc-376403무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGκ BP-HRP는 Notch1 항체 (A-8) WB 및 IHC(P) 애플리케이션용입니다. 이 시약은 현재 Notch1 항체 (A-8)(아래 주문 정보 참조)와 함께 번들로 제공됩니다. 추가 m-IgGκ BP 접합체는 마우스 IgG 결합 단백질 전체 목록을 참조하세요.

빠른 링크

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Notch 1 항체(A-8)는 마우스, 쥐 및 인간 유래의 Notch 1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgM κ 마우스 모노클로날 Notch 1 항체(NOTCH1 항체로도 지정됨)입니다. Notch 1 항체(A-8)는 비접합 항-노치 1 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-노치 1 항체 형태로도 사용할 수 있습니다. LIN-12/Notch 막 통과 수용체는 세포의 운명 결정을 조절하여 발달에 중심적인 역할을 하는 것으로 알려져 있습니다. 포유류에서 네 개의 노치 동족체(노치 1, 노치 2, 노치 3, 노치 4)가 확인되었습니다. 노치 유전자는 다양한 배아 및 성인 조직에서 발현되며, 이는 노치 유전자가 여러 신호 전달 경로에 관여한다는 것을 시사합니다. 노치 단백질은 인간 종양에서 과발현되거나 재배열된 것으로 밝혀졌습니다. 노치의 리간드에는 재기드1, 재기드2, 델타가 있습니다. 재기드1은 근육 조절 및 구조 유전자의 발현을 억제하여 노치를 활성화하고 근아세포 분화를 방지할 수 있습니다. 재기드2는 상피-중간엽 상호 작용에 의존하는 조직 발달에 관여할 수 있습니다. 부신과 태반에서 정상적으로 발현되는 것 외에도 델타 발현은 신경내분비 종양에서도 발견되었습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Notch1 항체 (A-8)

sc-376403
200 µg/ml
$316.00

Notch1 (A-8): m-IgGκ BP-HRP 번들

sc-552043
200 µg Ab; 40 µg BP
$354.00

Notch1 항체 (A-8) AC

sc-376403 AC
500 µg/ml, 25% agarose
$416.00

Notch1 항체 (A-8) Alexa Fluor® 488

sc-376403 AF488
200 µg/ml
$357.00

Notch1 항체 (A-8) Alexa Fluor® 647

sc-376403 AF647
200 µg/ml
$357.00

Notch1 항체 (A-8) FITC

sc-376403 FITC
200 µg/ml
$330.00

Notch1 항체 (A-8) HRP

sc-376403 HRP
200 µg/ml
$316.00

Notch1 (A-8) 중화펩타이드

sc-376403 P
100 µg/0.5 ml
$68.00

Notch1 항체 (A-8) PE

sc-376403 PE
200 µg/ml
$343.00

Hello, What secondary antibody should i use for WB? I can't see anywhere the host species,..Thanks

Asked by: myrto
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

Hello, I would like to use Notch-1 Antibody (A-8) primarily for western blot and later maybe for IC. Could you let me know if I could purchase the antibody conjugated to Alexa Fluor 488 and use it for WB Too or for WB only the HRP conjugated works?

Asked by: myrto
Yes, you can use conjugates to Alexa Fluor 488 for Western blotting.
Answered by: Tech Sales
Date published: 2021-03-15

Can I use this antibody for IF-P staining? If not which product can I use for IF tissue staining?

Asked by: JordanA
Thank you for your question. This antibody is recommended for IF and IHC(P), or immunofluorescence and immunohistochemistry on formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2019-07-18

I purchased this Notch 1 antibody (A-8) to do western blot for human breast cancer cell lines lysates, but I am not sure where the band will appear in the gel (110KD or 210 KD?). Please tell me a relatively accurate position. THX~

Asked by: Tianzi
Thank you for your question. The expected molecular weight for Notch one is ~300 kDa (precursor) and two possible cleaved forms which run around ~180 and ~115-120kDa.
Answered by: Tech Service
Date published: 2021-04-19

Hi, I am intersted in Notch 1 Antibody (A-8) Alexa Fluor® 647 (sc-376403 AF647, 314USD) I just saw its concentration is 200ug/mL. How about the volume? Thanks.

Asked by: Bin Zhao
Thank you for your question. It is provided as 1ml.
Answered by: Tech Service
Date published: 2018-11-21

I would like to use Notch 1 Antibody (A-8): sc-376403 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376403 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 5 out of 5 by from Notch1 worked very well in IHCNotch1 worked very well in IHC in human tissues by DAB staining
Date published: 2023-05-16
Rated 4 out of 5 by from Buon anticorpo per WB su campioni proteici umanitestato per western blot ad una concentrazione di 1:1000 su estratti proteici totali di cellule THP1 e su LX2 umane. tempo di esposizione rapido, banda netta
Date published: 2022-11-21
Rated 1 out of 5 by from No staining in murine heartThe antibody has been tested with different antigen retrival protocols. None of them worked out.
Date published: 2022-03-03
Rated 4 out of 5 by from Good for flow cytometryThis antibody works well for flow cytometry at 1:50 but requires permeabilization of the cell membrane.
Date published: 2019-02-26
Rated 4 out of 5 by from THIS IS A GOOD ANTIBODYI got a free sample of this antibody and it is working very fine. I am interested in buying a new vial.
Date published: 2018-03-29
Rated 4 out of 5 by from Antibody works greatBought this Notch1 monoclonal A8 Ab. Works better than Notch C-20 antibody. Specificity is good
Date published: 2018-02-08
Rated 3 out of 5 by from be patient !This antibodies is working just fine but for me I just need to wait and give it a little long exposure time..
Date published: 2017-12-21
Rated 2 out of 5 by from backgroundthe antibody was tested on rat tissue samples in western blot. A signal is detected but with a high background level
Date published: 2017-12-13
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Notch1 항체 (A-8) is rated 4.3 out of 5 by 27.
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