NHERF-1 Antibody (6): sc-136228
- NHERF-1 Antibody (6) is a mouse monoclonal IgG1, cited in 1 publications, provided at 50 µg/0.5 ml
- raised against amino acids 128-249 of NHERF-1 of human origin
- recommended for detection of NHERF-1 of human origin by WB, IP and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for NHERF-1 Antibody (6). This work is in progress.
NHERF-1 Antibody (6) is a mouse monoclonal IgG1 antibody that detects NHERF-1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). NHERF-1, also known as EBP50, plays a crucial role in regulating the Na+/H+ exchange protein NHE3, which is essential for transepithelial sodium absorption primarily in the intestinal and renal brush border membranes. NHERF-1′s ability to interact with NHE3 through two PDZ domains allows NHERF-1 to act as a scaffold for a multiprotein complex that includes Ezrin, facilitating cAMP-mediated phosphorylation and subsequent inhibition of NHE3 activity. This regulation is vital for maintaining sodium homeostasis and fluid balance in the body. Additionally, NHERF-1′s amino-terminal PDZ domain is important for dimerization in vivo, which further influences functional interactions. The phosphorylation of NHERF-1 at Ser 289 by G protein-coupled receptor kinase 6A highlights the intricate signaling pathways that modulate activity, while NHERF-2, another member of the NHERF family, also contributes to the regulation of sodium transport through NHE2. With a significant role in cellular signaling and ion transport, NHERF-1 is a key protein for researchers studying renal and intestinal physiology.
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NHERF-1 Antibody (6) References:
- Receptor activity-modifying protein (RAMP) isoform-specific regulation of adrenomedullin receptor trafficking by NHERF-1. | Bomberger, JM., et al. 2005. J Biol Chem. 280: 23926-35. PMID: 15805108
- Suppression of breast cancer cell growth by Na+/H+ exchanger regulatory factor 1 (NHERF1). | Pan, Y., et al. 2006. Breast Cancer Res. 8: R63. PMID: 17078868
- NHERF1 regulates parathyroid hormone receptor membrane retention without affecting recycling. | Wang, B., et al. 2007. J Biol Chem. 282: 36214-22. PMID: 17884816
- Dynamics of PTH-induced disassembly of Npt2a/NHERF-1 complexes in living OK cells. | Weinman, EJ., et al. 2011. Am J Physiol Renal Physiol. 300: F231-5. PMID: 21048030
- Fibroblast growth factor-23-mediated inhibition of renal phosphate transport in mice requires sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) and synergizes with parathyroid hormone. | Weinman, EJ., et al. 2011. J Biol Chem. 286: 37216-21. PMID: 21908609
- Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis. | Liu, L., et al. 2012. J Biol Chem. 287: 43312-21. PMID: 23109343
- Distribution dynamics and functional importance of NHERF1 in regulation of Mrp-2 trafficking in hepatocytes. | Karvar, S., et al. 2014. Am J Physiol Cell Physiol. 307: C727-37. PMID: 25163515
- PTEN inhibits macrophage polarization from M1 to M2 through CCL2 and VEGF-A reduction and NHERF-1 synergism. | Li, N., et al. 2015. Cancer Biol Ther. 16: 297-306. PMID: 25756512
- A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression. | Yang, X., et al. 2017. Anticancer Res. 37: 67-73. PMID: 28011475
- NHERF1 Enhances Cisplatin Sensitivity in Human Cervical Cancer Cells. | Tao, T., et al. 2017. Int J Mol Sci. 18: PMID: 28085111
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHERF-1 Antibody (6) | sc-136228 | 50 µg/0.5 ml | $322.00 |