NHERF-1 Antibody (4i337): sc-71697
- NHERF-1 Antibody (4i337) is a mouse monoclonal IgG2b provided at 100 µg/ml
- raised against recombinant full length NHERF-1 of human origin
- recommended for detection of NHERF of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for NHERF-1 Antibody (4i337). This work is in progress.
NHERF-1 Antibody (4i337) is a mouse monoclonal IgG2b antibody that detects NHERF-1 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. NHERF-1, also known as EBP50, plays a crucial role in regulating the Na+/H+ exchange protein NHE3, which is essential for transepithelial sodium absorption primarily in the intestinal and renal brush border membranes. Anti-NHERF-1 antibody (4i337) interacts with NHE3 through its two PDZ domains, allowing NHERF-1 to act as a scaffold for a multiprotein complex that includes Ezrin, facilitating cAMP-mediated phosphorylation and subsequent inhibition of NHE3 activity. This regulation is vital for maintaining sodium homeostasis and fluid balance in the body. Additionally, NHERF-1′s amino-terminal PDZ domain is important for its dimerization in vivo, which further influences its regulatory functions. The phosphorylation of NHERF-1 at Ser 289 by G protein-coupled receptor kinase 6A highlights the intricate signaling pathways that modulate its activity, while NHERF-1′s interaction with NHERF-2, also known as E3KARP, underscores the complexity of sodium transport regulation across different tissues.
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NHERF-1 Antibody (4i337) References:
- NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranes. | Weinman, EJ., et al. 2003. FEBS Lett. 536: 141-4. PMID: 12586353
- Expression of NHERF-1, NHERF-2, PDGFR-alpha, and PDGFR-beta in normal human kidneys and in renal transplant rejection. | Weinman, EJ., et al. Pathobiology. 70: 314-23. PMID: 12865627
- The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a. | Weinman, EJ., et al. 2005. J Physiol. 567: 27-32. PMID: 15932893
- NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. | Wheeler, D., et al. 2007. J Biol Chem. 282: 25076-87. PMID: 17599914
- NHERF-1 binds to Mrp2 and regulates hepatic Mrp2 expression and function. | Li, M., et al. 2010. J Biol Chem. 285: 19299-307. PMID: 20404332
- NHERF1 regulates gp120-induced internalization and signaling by CCR5, and HIV-1 production. | Kuang, YQ., et al. 2012. Eur J Immunol. 42: 299-310. PMID: 22028271
- NHERF-1 regulation of EGF and neurotensin signalling in HT-29 epithelial cells. | Kruger, WA., et al. 2013. Biochem Biophys Res Commun. 432: 568-73. PMID: 23454118
- Role of the PDZ-scaffold protein NHERF1/EBP50 in cancer biology: from signaling regulation to clinical relevance. | Vaquero, J., et al. 2017. Oncogene. 36: 3067-3079. PMID: 28068322
- Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation. | Kammala, AK., et al. 2020. Int J Mol Sci. 21: PMID: 33092043
- Nuclear expression of NHERF1/EBP50 in Clear Cell Renal Cell Carcinoma. | Lema, BE., et al. 2021. Acta Histochem. 123: 151717. PMID: 33965858
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHERF-1 Antibody (4i337) | sc-71697 | 100 µg/ml | $322.00 |