NG2 Antibody (7.1): sc-53508

NG2 Antibody (7.1) is a mouse monoclonal IgG1 antibody that detects NG2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). NG2, also known as melanoma-associated chondroitin sulfate proteoglycan 4, plays a crucial role in stabilizing cell-substratum interactions during the early stages of melanoma cell spreading on endothelial basement membranes, which is vital for tumor progression. The protein′s structure, characterized by unique chondroitin sulfate glycosaminoglycan chains, allows NG2 to interact with various extracellular matrix components and cell surface receptors, facilitating critical signaling pathways that promote tumor invasion and growth. Notably, phosphorylation at threonine 2256 in rat NG2 (threonine 2252 in human NG2) is essential for redistribution on the surface of astrocytomas, leading to enhanced cell polarization and increased motility, which are key factors in cancer metastasis. Additionally, NG2 serves as a co-receptor for α4β1 integrin, further underscoring its importance in malignant melanoma cell behavior. NG2 expression on blood vessels throughout the rat embryo and on endothelial cells in the central nervous system highlights its significance in vascular development and function. Furthermore, NG2 is recognized as a novel marker for epidermal stem cells, contributing to their organized distribution by promoting clustering, which is essential for maintaining tissue homeostasis and regeneration.

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NG2 Antibody (7.1) References:

1. Multipotency and therapeutic potential of NG2 cells.  |  Valny, M., et al. 2017. Biochem Pharmacol. 141: 42-55. PMID: 28522408
2. The regulatory mechanisms of NG2/CSPG4 expression.  |  Ampofo, E., et al. 2017. Cell Mol Biol Lett. 22: 4. PMID: 28536635
3. NG2/CSPG4 and progranulin in the posttraumatic glial scar.  |  Schäfer, MKE. and Tegeder, I. 2018. Matrix Biol. 68-69: 571-588. PMID: 29054751
4. NG2-glia crosstalk with microglia in health and disease.  |  Zhang, Z., et al. 2022. CNS Neurosci Ther. 28: 1663-1674. PMID: 36000202
5. Pathology-induced NG2 proteoglycan expression in microglia.  |  Meyer, E. and Scheller, A. 2023. Neural Regen Res. 18: 801-802. PMID: 36204842
6. Roles of NG2 Glia in Cerebral Small Vessel Disease.  |  He, Y., et al. 2023. Neurosci Bull. 39: 519-530. PMID: 36401147
7. The NG2-glia is a potential target to maintain the integrity of neurovascular unit after acute ischemic stroke.  |  Hu, X., et al. 2023. Neurobiol Dis. 180: 106076. PMID: 36921779
8. Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells.  |  Janeckova, L., et al. 2024. Glia. 72: 245-273. PMID: 37772368
9. Targeting NG2 relieves the resistance of BRAF-mutant thyroid cancer cells to BRAF inhibitors.  |  Sui, F., et al. 2024. Cell Mol Life Sci. 81: 238. PMID: 38795180
10. NG2-Glia Cause Diabetic Blood-Brain Barrier Disruption by Secreting MMP-9.  |  Li, X., et al. 2024. Diabetes Metab J.. PMID: 39043444

The preferred NG2 antibody is NG2 Antibody (LHM 2): sc-53389.