mouse origin의 Neurogenin 3에서 N-terminus에 위치한 15-49의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
안티-Neurogenin 3 항체 (C-7)는 WB, IP, IF and ELISA으로 mouse and rat유래의 Neurogenin 3 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Neurogenin 3 항체 (C-7)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Neurogenin 3 Antibody (C-7) for WB applications. This reagent is now offered in a bundle with Neurogenin 3 Antibody (C-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Neurogenin 3 Antibody (C-7) is a high quality monoclonal Neurogenin 3 antibody (also designated NGN3 antibody, BHLHa7 antibody, Class A Basic Helix-Loop-Helix Protein 7 antibody, Protein Atonal Homolog 5 antibody, Neurogenin-3 antibody, Math4B antibody, NGN-3 antibody, BHLHA7 antibody, or ATOH5 antibody) suitable for the detection of the Neurogenin 3 protein of mouse and rat origin. Neurogenin 3 Antibody (C-7) is available as both the non-conjugated anti-Neurogenin 3 antibody form, as well as multiple conjugated forms of anti-Neurogenin 3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the nuerogenin gene promoter. Neurogenin 3 is involved in the intial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, Neuro D, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of Neuro D in a bHLH cascade. Neurogenin 3 activates the expression of Neuro D at the onset of islet cell differentiation through box sequences E1 and E3 in the Neuro D promoter.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-374442 P appropriate for?
Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: