Nek1 CRISPR/Cas9 KO Plasmid (m): sc-421852
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nek1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nek1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nek1 Antibody (E-10): sc-398813
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nek1 CRISPR/Cas9 KO Plasmid (m) | sc-421852 | 20 µg | $397.00 |
Overview
Nek1 (NIMA related kinase 1) is a serine/threonine kinase that coordinates key aspects of cell cycle control, centrosome and cilia homeostasis, and microtubule-associated processes. In mouse cells, Nek1 has been linked to DNA damage signaling and repair, contributing to genome stability through regulation of checkpoint and repair pathways. Its functions intersect with ciliogenesis and cytoskeletal organization, connecting Nek1 activity to developmental and tissue maintenance programs. Dysregulation of Nek1-associated pathways has been implicated in cilia-related pathobiology and neurodegeneration-relevant mechanisms, making it a useful target for dissecting stress responses and cell architecture phenotypes.
Nek1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nek1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nek1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nek1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nek1 protein expression.
This CRISPR knockout system enables efficient generation of Nek1-deficient cell models for investigation of Nek1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nek1 exon(s) critical for Nek1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nek1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nek1 CRISPR/Cas9 KO Plasmid (m) and Nek1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nek1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nek1 HDR Plasmid (m) and Nek1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nek1 homology arms to support homology-directed repair at defined Nek1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.