specific for an epitope mapping between amino acids 91-108 within a cytoplasmic domain of NCKX5 of human origin
NCKX5 항체 (A-6)는 WB, IP, IF and ELISA으로 human유래의 NCKX5 를 감지하는 데에 추천한다 .
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-NCKX5 항체 (A-6)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for NCKX5 Antibody (A-6) for WB applications. This reagent is now offered in a bundle with NCKX5 Antibody (A-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
NCKX5 Antibody (A-6) is a high quality monoclonal NCKX5 antibody (also designated NCKX5 antibody) suitable for the detection of the NCKX5 protein of human origin. NCKX5 Antibody (A-6) is available as both the non-conjugated anti-NCKX5 antibody form, as well as multiple conjugated forms of anti-NCKX5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Plasma membrane sodium/calcium exchangers are crucial for the maintenance of intracellular calcium homeostasis and the modulation of electrical conduction. Potassium-dependent sodium/calcium exchangers, such as NCKX5 (Na(+)/K(+)/Ca(2+)-exchange protein 5), presumably transport an intracellular calcium and a potassium ion in exchange for four extracellular sodium ions. NCKX5, also known as JSX, SHEP4 or SLC24A5, is a 500 amino acid multi-pass membrane protein belonging to the sodium/potassium/calcium exchanger family and the SLC24A subfamily. NCKX5 is expressed in skin, retinal epithelium and brain. Considered a cation exchanger, NCKX5 may be involved in skin pigmentation by participating in ion transport in melanosomes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
For Western Blot, is it recommended to use denatured or non-denatured conditions with NCKX5 (A-6): sc-515715 monoclonal antibody?
Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.