Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
myoglobin Double Nickase Plasmid (m): sc-421575-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- myoglobin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- myoglobin Double Nickase Plasmid (m) and myoglobin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mb. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: myoglobin Antibody (A-6): sc-393020
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
myoglobin Double Nickase Plasmid (m) | sc-421575-NIC | 20 µg | $410.00 | |||
myoglobin Double Nickase Plasmid (m2) | sc-421575-NIC-2 | 20 µg | $410.00 |
Mouse Mb encodes myoglobin, a heme-containing oxygen-binding protein enriched in cardiac and oxidative skeletal muscle where it supports intracellular oxygen storage and diffusion. By buffering oxygen availability, myoglobin helps sustain mitochondrial oxidative phosphorylation and limits hypoxia-associated metabolic stress during fluctuating workload. Mb expression and myoglobin redox chemistry intersect with nitric oxide and reactive oxygen species handling, influencing muscle energetics and vasoregulation. Altered myoglobin abundance or function is frequently studied in contexts of ischemia, cardiomyopathy, and exercise adaptation to understand muscle oxygen homeostasis and stress responses.
myoglobin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mb locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mb. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mb function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mb-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.