Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MYBPC3 Double Nickase Plasmid (h): sc-401409-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MYBPC3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MYBPC3 Double Nickase Plasmid (h) and MYBPC3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MYBPC3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MYBPC3 Antibody (G-7): sc-137237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYBPC3 Double Nickase Plasmid (h) | sc-401409-NIC | 20 µg | $410.00 | |||
MYBPC3 Double Nickase Plasmid (h2) | sc-401409-NIC-2 | 20 µg | $410.00 |
MYBPC3 encodes cardiac myosin-binding protein C, a sarcomeric thick-filament accessory protein that modulates actin–myosin cross-bridge cycling and contributes to the stabilization and regulation of contractile function. Through phosphorylation-dependent interactions with myosin and titin, MYBPC3 helps tune calcium-sensitive contractility and supports orderly assembly and maintenance of the cardiac sarcomere. Altered MYBPC3 expression or function perturbs myofibrillar organization and myocardial mechanics, linking this gene to inherited cardiomyopathy phenotypes and cardiac remodeling pathways. As a human cardiac structural gene, MYBPC3 is widely studied in models of sarcomere biology, mechanotransduction, and cardiomyocyte maturation.
MYBPC3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MYBPC3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MYBPC3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MYBPC3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MYBPC3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.