Date published: 2025-9-6

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MRTF-A/MKL1 항체 (G-7): sc-398675

4.0(10)
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데이터 시트
  • MRTF-A/MKL1 항체 G-7 는 마우스 monoclonal IgM κ MRTF-A/MKL1 항체, 18간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 MRTF-A의 내부에 위치한 905-931의 아미노산에 해당됩니다.
  • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 MRTF-A을 검출할것을 권장합니다.
  • MRTF-A/MKL1 (G-7): sc-398675무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 MRTF-A/MKL1 항체(G-7)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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MRTF-A 항체(G-7)는 마우스, 쥐 및 인간 유래의 MRTF-A 단백질을 WB, IP, IF 및 ELISA로 검출하는 IgM κ 마우스 단일 클론 MRTF-A 항체(MKL1 항체, MRTFA 항체 또는 심근 관련 전사 인자 A 항체라고도 함)입니다. MRTF-A 항체(G-7)는 비접합 항-MRTF-A 항체와 다중 Alexa Fluor® 접합체 모두로 제공됩니다. 혈청 반응 인자(SRF)는 성장 자극에 대한 많은 세포 유전자의 일시적 반응을 매개하는 서열인 혈청 반응 요소(SRE)에 결합하는 전사인자입니다. SRF는 성장 인자에 반응하여 여러 근육 유전자의 일시적인 반응을 조절하고 보조 근육 인자를 모집하여 이러한 근육 유전자를 활성화합니다. SRF는 심혈관계의 기원으로 이어지는 척추동물 중배엽의 형성에 필요합니다. 미오카딘은 심장 근육 세포에서 SRF와 결합하여 심장 근육 프로모터를 활성화합니다. MKL1이라고도 하는 미오카딘 관련 전사인자 A(MRTF-A)는 SRF와 상호 작용하여 전사 활동을 자극합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

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MRTF-A/MKL1 항체 (G-7)

sc-398675
200 µg/ml
$316.00

MRTF-A/MKL1 (G-7) 중화펩타이드

sc-398675 P
100 µg/0.5 ml
$68.00

Do you recommend G-7 or G-8 for IHC from 4% PFA fixed paraffin embedded tissue on 5 micron sections?

Asked by: MoneyShot
Thank you for your question. I would suggest sc-398675 for IHC use, although either one should be perfectly fine.
Answered by: Tech Service
Date published: 2019-10-18

What secondary antibody do you suggest to use? Your data sheet says it's IgM light chain specific, all mouse IgM HRP I could find are specific for the heavy chain. However your data sheet recommends mouse IgG light chain specific HRP--very confusing......

Asked by: LiUTMDACC
Thank you for your question. We recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP), m-IgGκ BP-HRP: sc-516102 (see link below). We do not offer a secondary binding protein that is specific to mouse IgM. However, our in-house data indicates that this anti-mouse IgG kappa light chain binding protein will also bind to the kappa light chain of mouse IgM. You could also use any conventional anti-mouse IgM secondary antibody. We do not offer any anti-mouse IgM that is specific for the heavy chain, so I'm not sure what you are referring to, unless those are provided by other suppliers. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-06-22

Can you recommend a positive control to use with MRTF-A (G-7): sc-398675 monoclonal antibody for Western blot?

Asked by: jenni
We recommend Hep G2 (sc-2227), HEK293 (sc-45136), HUV-EC-C (sc-364180) or CCRF-CEM (sc-2225) whole cell lysates or HeLa nuclear extract (sc-2120) to use as positive controls as we have Western blot data showing specific detection of MRTF-A in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-17
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Rated 5 out of 5 by from Detection of endogenous MRTF-A/MKL1used for detection of endogenous MRTF-A/MKL1 by immunoblotting
Date published: 2022-05-25
Rated 5 out of 5 by from Great fzqtureI bought this one month ago and it is the Best thing!
Date published: 2021-06-01
Rated 4 out of 5 by from MRTF-A抗体评论我一个月前买了该抗体,效果还不错,虽然条带不单一,有很多杂带,但是目的蛋白条带还是很亮,没有其他问题
Date published: 2017-11-07
Rated 5 out of 5 by from 不错申请到了公司的小样,很好用,按照常规RIPA裂解,上样,以前得到的条带都是多条,换抗体后得到了单一的条带。
Date published: 2017-08-27
Rated 5 out of 5 by from This antibody works great for meI used this antibody for the detection of MRTF-A protein in 4T1 mouse mamary tumor cell nuclear lysate
Date published: 2017-05-22
Rated 3 out of 5 by from Unsure of specificityIt stains in the correct cellular area but its cellular localization is unspecific. I stained a fibrotic Human and Mouse Skin, liver and Kidney tissue. Staining is specific. However, I did not do an in vitro actin assay to truly determine antibody specificity. MRTF-A is published to be susceptible to actin destabilizing drugs.
Date published: 2017-04-22
Rated 1 out of 5 by from Theona NatisviliThe antibody does not work for ChIP, did not give any signal.
Date published: 2017-04-07
Rated 5 out of 5 by from Clean bands for WBMRTF-A (G-7) gives nice clean bands for Western blot at 160 kDa. Recommend NIH/3T3 Whole Cell Lysate: sc-2210 as a positive control.
Date published: 2017-01-13
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MRTF-A/MKL1 항체 (G-7) is rated 4.0 out of 5 by 10.
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