MPO light chain/Myeloperoxidase Antibody (A-5): sc-365436

MPO light chain Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MPO light chain of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MPO (A-5) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heme protein myeloperoxidase (MPO) plays a crucial role in immune response, primarily found in azurophilic granules of neutrophils and polymorphonuclear leukocytes. MPO is essential for optimal oxygen-dependent microbiocidal activity, catalyzing the production of hypochlorous acid and other reactive oxygen species vital for killing pathogens. MPO synthesis begins during promyelocytic stage of myeloid differentiation, where precursor undergoes cotranslational N-linked glycosylation, resulting in a glycoprotein processed and transported to lysosomes. Processing involves glucosidases in endoplasmic reticulum or early cis Golgi, converting pro-MPO into a form sorted into prelysosomal compartment, leading to proteolytic maturation of native MPO, existing as a dimer in normal neutrophils. Calreticulin, a calcium-binding protein in endoplasmic reticulum, specifically interacts with fully glycosylated apopro-MPO, highlighting post-translational modifications importance in functionality. MPO mRNA is abundantly expressed in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells, with high levels found in circulating neutrophils and monocytes, while remaining undetectable in microglia, brain-specific macrophages, or normal brain tissue.

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MPO light chain/Myeloperoxidase Antibody (A-5) References:

1. Functional activity of three distinct myeloperoxidase (MPO) promoters in human myeloid cells.  |  Lin, KM. and Austin, GE. 2002. Leukemia. 16: 1143-53. PMID: 12040446
2. Neutrophil functions of patients with MPO-ANCA-related vasculitis.  |  Suzuki, K. 2003. Intern Med. 42: 552-3. PMID: 12879944
3. Human hemi-myeloperoxidase. Initial chlorinating activity at neutral pH, compound II and III formation, and stability towards hypochlorous acid and high temperature.  |  Zuurbier, KW., et al. 1992. Eur J Biochem. 205: 737-42. PMID: 1315274
4. Incidence of PR3- and MPO-ANCA autoantibody specificity changes in ANCA-associated vasculitis.  |  Holding, S., et al. 2015. Ann Clin Biochem. 52: 297-301. PMID: 24723621
5. Preparation and characterization of monoclonal antibodies against human myeloperoxidase.  |  Homma, T., et al. 1989. Arch Biochem Biophys. 273: 189-96. PMID: 2547339
6. Posttranslational processing of a human myeloid lysosomal protein, myeloperoxidase.  |  Nauseef, WM. 1987. Blood. 70: 1143-50. PMID: 2820530
7. Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation.  |  Nauseef, WM., et al. 1988. Eur J Haematol. 40: 97-110. PMID: 2831080
8. Molecular cloning and characterization of cDNA for human myeloperoxidase.  |  Morishita, K., et al. 1987. J Biol Chem. 262: 3844-51. PMID: 3029127
9. Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species.  |  Johnson, KR., et al. 1987. Nucleic Acids Res. 15: 2013-28. PMID: 3031585
10. Discovery of a novel indole pharmacophore for the irreversible inhibition of myeloperoxidase (MPO).  |  Patnaik, A., et al. 2020. Bioorg Med Chem. 28: 115548. PMID: 32503688
11. Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase.  |  Nauseef, WM., et al. 1995. J Biol Chem. 270: 4741-7. PMID: 7876246
12. Myeloperoxidase polymorphism is associated with gender specific risk for Alzheimer's disease.  |  Reynolds, WF., et al. 1999. Exp Neurol. 155: 31-41. PMID: 9918702

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MPO light chain/Myeloperoxidase Antibody (A-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MPO light chain/Myeloperoxidase Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MPO light chain/Myeloperoxidase Antibody (A-5) Specifications

Alternate Product Name: Myeloperoxidase (MPO) light chain

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 165-278

Epitope Target: MPO

Epitope Species: human

Product Reactivity/Detection: MPO light chain

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MPO light chain/Myeloperoxidase Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MPO/Myeloperoxidase antibody is MPO/Myeloperoxidase Antibody (266-6K1): sc-52707.