Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MOB2 CRISPR/Cas9 KO Plasmid (h): sc-413424
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOB2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MOB2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MOB2 Antibody (2400C3a): sc-81564
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOB2 CRISPR/Cas9 KO Plasmid (h) | sc-413424 | 20 µg | $397.00 |
Overview
MOB2 (MOB kinase activator 2) is a conserved adaptor protein that interacts with NDR/LATS family kinases to modulate Hippo-related signaling, influencing phosphorylation cascades that govern cell proliferation, apoptosis, and cytoskeletal organization. Through regulation of kinase activity and subcellular localization of pathway components, MOB2 contributes to control of cell-cycle progression, polarity, and contact-dependent growth. Dysregulated Hippo/NDR signaling is frequently implicated in oncogenic phenotypes, and altered MOB2 function has been associated with perturbations in tumor suppressor networks and proliferative control. MOB2 is therefore relevant for studying mechanisms linking kinase regulation to tissue homeostasis and disease-associated signaling rewiring.
MOB2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MOB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MOB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MOB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MOB2 protein expression.
This CRISPR knockout system enables efficient generation of MOB2-deficient cell models for investigation of MOB2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MOB2 exon(s) critical for MOB2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MOB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MOB2 CRISPR/Cas9 KO Plasmid (h) and MOB2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MOB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MOB2 HDR Plasmid (h) and MOB2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MOB2 homology arms to support homology-directed repair at defined MOB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.