Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mitofilin CRISPR/Cas9 KO Plasmid (m): sc-429376
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mitofilin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Mitofilin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mitofilin Antibody (D-3): sc-390707
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mitofilin CRISPR/Cas9 KO Plasmid (m) | sc-429376 | 20 µg | $397.00 |
Overview
Mouse Immt encodes mitofilin, a core component of the MICOS (mitochondrial contact site and cristae organizing system) complex that maintains inner mitochondrial membrane architecture and crista junction formation. Mitofilin supports mitochondrial ultrastructure, coordinates contacts between the inner and outer membranes, and influences protein import and respiratory chain organization important for oxidative phosphorylation. Disruption of IMMT perturbs cristae morphology, mitochondrial dynamics, and bioenergetic homeostasis, processes that are widely implicated in neurodegeneration, cardiomyopathy, metabolic dysfunction, and cancer cell adaptation. As a nexus of mitochondrial structure–function coupling, Immt is frequently studied in pathways governing apoptosis sensitivity, mitophagy, and cellular stress responses.
Mitofilin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Immt gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Immt together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Immt open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Mitofilin protein expression.
This CRISPR knockout system enables efficient generation of Immt-deficient cell models for investigation of Mitofilin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Immt exon(s) critical for Mitofilin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Immt genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Mitofilin CRISPR/Cas9 KO Plasmid (m) and Mitofilin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Immt locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Mitofilin HDR Plasmid (m) and Mitofilin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Immt homology arms to support homology-directed repair at defined Immt target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.