Metaxin 2 Double Nickase Plasmid (m): sc-424699-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Metaxin 2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Metaxin 2 Double Nickase Plasmid (m) and Metaxin 2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mtx2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Metaxin 2 Antibody (H-2): sc-514231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Metaxin 2 Double Nickase Plasmid (m) | sc-424699-NIC | 20 µg | $410.00 | |||
Metaxin 2 Double Nickase Plasmid (m2) | sc-424699-NIC-2 | 20 µg | $410.00 |
Mouse Mtx2 encodes metaxin 2, an outer mitochondrial membrane protein that contributes to mitochondrial protein import by supporting assembly and function of the translocase of the outer membrane (TOM) machinery. Metaxin 2 helps maintain mitochondrial proteostasis, membrane integrity, and efficient biogenesis, thereby influencing oxidative phosphorylation and broader cellular energy metabolism. Perturbation of mitochondrial import pathways can drive stress responses, altered apoptosis susceptibility, and secondary effects on lipid metabolism and innate signaling. As a component of mitochondrial homeostasis, MTX2 is routinely studied in models of mitochondrial dysfunction relevant to neurodegeneration, metabolic phenotypes, and cellular stress sensitivity.
Metaxin 2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mtx2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mtx2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mtx2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mtx2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.