Date published: 2025-9-6

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MCT4 항체 (D-1): sc-376140

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데이터 시트
  • MCT4 항체 D-1 는 마우스 monoclonal IgG2a κ MCT4 항체, 83간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노 산376-465 mapping within a C-terminal cytoplasmic domain of MCT4 of human 의하고 기원한다
  • MCT4 항체 (D-1)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 MCT4 를 감지하는 데에 추천한다.
  • 항-MCT4 항체(D-1)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • MCT4 (D-1): sc-376140무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGκ BP-HRP는 MCT4 항체 (D-1) WB 및 IHC(P) 애플리케이션용입니다. 이 시약은 현재 MCT4 항체 (D-1)(아래 주문 정보 참조)와 함께 번들로 제공됩니다. 추가 m-IgGκ BP 접합체는 마우스 IgG 결합 단백질 전체 목록을 참조하세요.

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MCT4 항체(D-1)는 마우스, 쥐 및 인간 유래의 MCT4 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2a κ 마우스 모노클로날 MCT4 항체(SLC16A3 항체, 용질 운반체군 16 멤버 3 항체 또는 MCT3 항체로도 지정됨)입니다. MCT4 항체(D-1)는 비접합 항-MCT4 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-MCT4 항체 모두로 제공됩니다. 젖산염과 피루브산과 같은 단카르복실산염은 세포 대사에 필수적인 역할을 합니다. 젖산은 해당 작용의 결과로 대량으로 생성되며, 정상적인 생리적 조건에서 세포에 대부분의 ATP를 공급합니다. 그러나 젖산이 축적되면 세포 내 pH가 감소하고 해당 작용이 중단됩니다. 해당 작용이 빠른 속도로 계속되려면 젖산을 세포 밖으로 운반해야 합니다. 이 수송 과정은 양성자 심포트 역할을 하며 L-락테이트에 대해 입체 선택적인 모노카복실레이트 수송체(MCT) 계열에 의해 수행됩니다. MCT 계열은 세포질에 위치한 아미노 및 카르복시 말단이 있는 10~12개의 막 통과 나선형(TM) 도메인을 포함하는 최소 8개의 멤버인 MCT1~8로 구성됩니다. MCT1은 종양 세포와 적혈구에서 광범위하게 발현되며 주요 형태의 MCT입니다. MCT2는 간과 고환에서 많이 발현되는 반면, MCT3와 MCT4는 골격근에서 주로 발현됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

MCT4 항체 (D-1) 참고문헌:

  1. 골격근에서의 젖산염 수송 - 모노카복실레이트 수송체의 역할과 조절.  |  Juel, C. and Halestrap, AP. 1999. J Physiol. 517 (Pt 3): 633-42. PMID: 10358105
  2. 양성자 연결 모노카복실레이트 수송체(MCT) 계열: 구조, 기능 및 조절.  |  Halestrap, AP. and Price, NT. 1999. Biochem J. 343 Pt 2: 281-99. PMID: 10510291
  3. 심장 및 골격근의 젖산 수송체(MCT 단백질)입니다.  |  Bonen, A. 2000. Med Sci Sports Exerc. 32: 778-89. PMID: 10776897
  4. 심장과 빠르게 수축하는 골격근에서 MCT1과 MCT4의 풍부함과 세포 내 분포.  |  Bonen, A., et al. 2000. Am J Physiol Endocrinol Metab. 278: E1067-77. PMID: 10827010
  5. 성체 및 젖먹이 쥐에서 뇌 내피와 신경교세포에 의한 모노카복실레이트 수송체 MCT1의 발현.  |  Gerhart, DZ., et al. 1997. Am J Physiol. 273: E207-13. PMID: 9252498
  6. 심근 허혈과 관련된 심장의 젖산염 수송.  |  Halestrap, AP., et al. 1997. Am J Cardiol. 80: 17A-25A. PMID: 9293952
  7. 새로운 포유류 모노카복실레이트 수송체(MCT) 동족체 4종의 복제 및 염기서열 분석 결과, 고대부터 존재했던 수송체 계열의 존재가 확인되었습니다.  |  Price, NT., et al. 1998. Biochem J. 329 (Pt 2): 321-8. PMID: 9425115
  8. 인간 모노카르복실레이트 수송체 2(MCT2)는 고친화성 피루브산염 수송체입니다.  |  Lin, RY., et al. 1998. J Biol Chem. 273: 28959-65. PMID: 9786900

주문정보

제품명카탈로그 번호 단위가격수량관심품목

MCT4 항체 (D-1)

sc-376140
200 µg/ml
$316.00

MCT4 (D-1): m-IgGκ BP-HRP 번들

sc-522851
200 µg Ab, 40 µg BP
$354.00

MCT4 항체 (D-1) AC

sc-376140 AC
500 µg/ml, 25% agarose
$416.00

MCT4 항체 (D-1) HRP

sc-376140 HRP
200 µg/ml
$316.00

MCT4 항체 (D-1) FITC

sc-376140 FITC
200 µg/ml
$330.00

MCT4 항체 (D-1) PE

sc-376140 PE
200 µg/ml
$343.00

MCT4 항체 (D-1) Alexa Fluor® 488

sc-376140 AF488
200 µg/ml
$357.00

MCT4 항체 (D-1) Alexa Fluor® 546

sc-376140 AF546
200 µg/ml
$357.00

MCT4 항체 (D-1) Alexa Fluor® 594

sc-376140 AF594
200 µg/ml
$357.00

MCT4 항체 (D-1) Alexa Fluor® 647

sc-376140 AF647
200 µg/ml
$357.00

MCT4 항체 (D-1) Alexa Fluor® 680

sc-376140 AF680
200 µg/ml
$357.00

MCT4 항체 (D-1) Alexa Fluor® 790

sc-376140 AF790
200 µg/ml
$357.00

FCM用にsc-376140 AF488を購入したのですが、こちらについては検証されていないのでしょうか?MCT4 (D-1) is available conjugated to Alexa Fluor® 488 (sc-376140 AF488) for WB (RGB), IF, IHC(P) and FC.とあるのですが。

Asked by: Tomoya
Thank you for your product review.Please contact our Technical Service Department of Japan for the consultation optimization suggestion and more information. 商品の問題、又は質問がございましたら、日本のディーラーを連絡し、お尋ねください。宜しくお願い致します。
Answered by: Jiajun Z
Date published: 2024-11-07

I am planning to use this antibody for detecting mouse MCT4 by FCM. Can you confirm that this antibody specifically targets the cytoplasmic domain of MCT4 and is suitable for use in both mouse and human samples?

Asked by: Tomoya
Thank you for your question. The MCT4 Antibody (D-1): sc-376140 has not been tested for FCM and is not recommended for FCM at this time. 質問ありがとうございます。なお、このmct4抗体(d-1) sc-376140については、fcm用に試験しておりませんので、現時点では推奨しておりません。
Answered by: Yanru Tao
Date published: 2024-11-05

MCT-4抗体の10 μgの無料サンプルを頂きIHCをお試したいのですが、いかがでしょうか。

Asked by: RyosukeY
質問頂き、誠にありがとうございます。抗体の購入とサンプル品の申請は日本のディーラーを連絡し、お尋ねください。宜しくお願い致します。 https://www.scbt.com/ja/resources/distributors?&_requestid=1655 Thank you very much for your question. Please contact your Japanese dealer and ask them to purchase antibodies and apply for sample products. Thank you for your cooperation. https://www.scbt.com/ja/resources/distributors?&_requestid=1655
Answered by: Jiajun Z
Date published: 2024-09-20

Which specific antigen retrieval do you recommand using MCT4 (D-1): sc-376140 antibody between :10 mM sodium citrate buffer, pH 6.0 (sc-294091); 50 mM glycine-HCl buffer (sc-29096), pH 3.5, and 0.01% (w/v) EDTA (sc-29092)?

Asked by: Nina 44
Thank you for your question. For antigen retrieval in experiments with this antibody we recommend heat treatment: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2022-06-22

Which specific antigen retrieval do you recommand using MCT4 (D-1): sc-376140 antibody for IHC(P)? Your protocol (immunoperoxidase-staining Antigen retrieval is described in step C) does not specify for this antibody specifically.

Asked by: Nina 44
Thank you for your question. For antigen retrieval in experiments with this antibody we recommend heat treatment: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2022-06-21

Can MCT4 antibody (D-1): sc-376140 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections? Which antigen retrieval do you recommand?

Asked by: Nina 44
Thank you for your question. sc-376140 is recommended to be used in IHC(P). You can find a detailed protocol by following the link below: https://www.scbt.com/resources/protocols/immunoperoxidase-staining Antigen retrieval is described in step C.
Answered by: Tech Support Europe
Date published: 2022-06-21

Would it be possible to purchase a sample of this antibody? MCT4 Antibody (D-1): sc-376140

Asked by: LCos
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service 1
Date published: 2021-01-28

I tried on mouse sample (4T1 cells lysate ) for WB, but doesn't work. is it possible to have the an aliquot of the discontinued MCT4 (sc-50329) that was working in my previous experiments?

Asked by: AnnaTo
Thank you for your question. The rabbit polyclonal antibody, MCT4 (H-90): sc-50329, has been discontinued since 2016, and is no longer available for sale or distribution. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2022-06-21
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Rated 5 out of 5 by from 功能很好我们团队在此买过抗体,说是功能很好,现在也推荐我来买了,看看这一次功能怎么样,用过一段时间之后我再来评论。
Date published: 2025-03-03
Rated 4 out of 5 by from Works wellUsed in WB and the clear band was detected. it worked well with rat samples.
Date published: 2023-06-21
Rated 3 out of 5 by from Worked for mouse proteinThis antibody seemed to pick up only the mouse protein, as opposed to a different one I tried before that only provided signal for the human cell line. At least I know they both express it!
Date published: 2022-01-05
Rated 5 out of 5 by from GoodUsed on HeLa, PC-3 and MCF7 cells, dil 1:1000, both Western blotting and immunofluorescence. Works well
Date published: 2021-06-24
Rated 5 out of 5 by from WonderfulThis is a very stable antibody, and the price is very good
Date published: 2020-09-02
Rated 5 out of 5 by from good for IHC and wester blottingStaining of 10um lung tissue slices looked good following concentrations suggested by SCBT. For Western blotting, 1/000 dillution is enough.
Date published: 2019-06-09
Rated 1 out of 5 by from no bandsno bands being detected in human melanoma cells, even the positive control was negative
Date published: 2019-04-16
Rated 5 out of 5 by from Anticuerpo con buen resultado por WBHemos obtenido unos resultados buenos en WB usando una concentración 1/1000. Se obtiene una banda es fuerte y específica.
Date published: 2019-03-18
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MCT4 항체 (D-1) is rated 4.1 out of 5 by 28.
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