Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCPIP Double Nickase Plasmid (h): sc-401790-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCPIP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MCPIP Double Nickase Plasmid (h) and MCPIP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZC3H12A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCPIP Antibody (H-6): sc-515275
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCPIP Double Nickase Plasmid (h) | sc-401790-NIC | 20 µg | $410.00 | |||
MCPIP Double Nickase Plasmid (h2) | sc-401790-NIC-2 | 20 µg | $410.00 |
ZC3H12A encodes MCPIP (also known as Regnase-1), an RNase and deubiquitinase that integrates post-transcriptional and ubiquitin signaling to restrain inflammatory gene expression. MCPIP promotes decay of select cytokine and immune-response mRNAs and modulates NF-κB and MAPK pathway outputs, shaping macrophage activation, T cell responses, and stress-induced transcriptional programs. By controlling RNA stability and signaling protein ubiquitination, MCPIP contributes to immune homeostasis and can influence chronic inflammation–associated phenotypes observed in cancer, autoimmunity, and cardiometabolic disease research contexts. Human MCPIP is therefore frequently studied in innate immune signaling, RNA turnover, and inflammation-driven cellular remodeling.
MCPIP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZC3H12A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZC3H12A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZC3H12A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZC3H12A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.