Saccharomyces cerevisiae origin의 Mcm2에서 N-terminus에 위치한 11-34의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
Mcm2 항체 (C-3)는 WB, IP, IF and ELISA으로 S. cerevisiae유래의 Mcm2 를 감지하는 데에 추천한다 .
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Mcm2 항체 (C-3)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Mcm2 Antibody (C-3) for WB applications. This reagent is now offered in a bundle with Mcm2 Antibody (C-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Orc1 and Orc2 (also designated Rrr1 or Sir5) are two of the six subunits that compose the yeast origin of replication complex (ORC). This complex binds to autonomously replicating sequences (ARS) and serves as an initiator protein for DNA replication. The minichromosome maintenance (Mcm) proteins also play an essential role in regulating DNA replication by binding to chromatin and activating the ORC-ARS complex (2-6). Cdc6, involved in limiting DNA replication to once per cell cycle, binds to the ORC and is essential for the assembly of the Mcm proteins. The transcription factor Abf1 (also designated Obf1 or Baf1) also binds to the ARS, and plays a role in gene silencing as well as in DNA replication.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with Mcm2 (C-3): sc-515723 monoclonal antibody for Western blot?
Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
Rated 2 out of
Date published: 2018-03-11
Rated 2 out of
not a great antibody for WBThis antibody generates a very weak (barely detectable) signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). Perhaps loading more extract would be helpful