Mcm2 항체 (C-3) WB, IP, IF 와ELISA으로 S. cerevisiae origin의 Mcm2을 검출할것을 권장합니다.

Mcm2 항체 (C-3): sc-515723

Mcm2 항체 (C-3) is rated 2.0 out of 5 by 2.
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데이터 시트

    • Mcm2 항체(C-3) 는 마우스 monoclonal IgG2b(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • Saccharomyces cerevisiae origin의 Mcm2에서 N-terminus에 위치한 11-34의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
    • Mcm2 항체 (C-3)는 WB, IP, IF and ELISA으로 S. cerevisiae유래의 Mcm2 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Mcm2 항체 (C-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Mcm2 (C-3): sc-515723무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Mcm2 Antibody (C-3) for WB applications. This reagent is now offered in a bundle with Mcm2 Antibody (C-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What blocking solution should I use with Mcm2 (C-3): sc-515723 monoclonal antibody for Western blot?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 2 out of 5 by from 跑出来带比较浅,跟说明书不太一样 试用装跑出来带比较浅,跟说明书不太一样,就一个条带 30ug蛋白,5%脱脂牛奶封闭,一抗过夜孵育,二抗牛奶室温两个小时
Date published: 2018-03-11
Rated 2 out of 5 by from not a great antibody for WB This antibody generates a very weak (barely detectable) signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). Perhaps loading more extract would be helpful
Date published: 2017-09-18
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