MBP 항체 (F-6) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 MBP isoforms 1, 3, 4, 5 and 6, (also designated Golli-MBP1, MBP1, MBP2, MBP3 and MBP4)을 검출할것을 권장합니다.

MBP 항체 (F-6): sc-271524

MBP 항체 (F-6) is rated 4.6 out of 5 by 23.
  • y_2021, m_8, d_4, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_ko_KR, sid_271524, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
loading
5
17
4
4
3
1
2
0
1
1

데이터 시트

    • 안티-MBP 항체 F-6 는 마우스 monoclonal IgG1 κ MBP 항체, 31간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 MBP에서 에 가까운196-237의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
    • 안티-MBP 항체 (F-6)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 MBP isoforms 1, 3, 4, 5 and 6, (also designated Golli-MBP1, MBP1, MBP2, MBP3 and MBP4) 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-MBP 항체 (F-6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • WB, IHC(P) 와 ELISA에 사용가능한 biotin결합제품 이 있습니다.
    • MBP (F-6): sc-271524무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MBP Antibody (F-6) for WB and IHC(P) applications. These reagents are now offered in bundles with MBP Antibody (F-6) (see ordering information below).
loading

Can this antibody be used for FACS of rat oligodendrocytes? 

Asked by: Babio
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2018-12-11

is this antibody suitable for IF staining of human oligodendrocytes?

Asked by: alinas
Thank you for your question. Yes, this antibody is recommended for IF on human samples, so we warranty it for that use. We have not specifically tested it on oligodendrocytes in house. Please reach out to Technical Support if you have any further questions.
Answered by: Tech Service
Date published: 2017-11-21

Can this antibody be used successfully to stain mouse tissue in IHC(P)?

Asked by: AbPolly
Thank you for your question. Yes, this antibody is appropriate for IHC of paraffin-embedded mouse tissue. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 7
Date published: 2017-01-11

Is this antibody recommended for detection of all isoforms of MBP?

Asked by: AbPolly
Thank you for your question. This MBP antibody is recommended for detection of all isoforms of MBP except for isoform 2, which has a sequence change at the epitope site.
Answered by: Technical Support 7
Date published: 2017-01-11
  • y_2021, m_8, d_4, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_ko_KR, sid_271524, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 132ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from works well for western blot Tried this at 1:200 dilution for western blot, got a clear band.
Date published: 2020-06-05
Rated 5 out of 5 by from very good antibody Tested by Westerb blot on P17 rat cerebellar lysate. 1:100 dilution O/N in 3% BSA 0.2% Tween TBS (1:300 secondary Ab, 2h RT). Strong signal (dilution required), specific.
Date published: 2020-02-12
Rated 5 out of 5 by from Great product! It work so nice in Westerns that I want to buy now the conjugated antibody for IHC
Date published: 2019-12-03
Rated 5 out of 5 by from works well this antibody works well in WB with 1:5000 dilution
Date published: 2019-10-03
Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from Great antibody ! 4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked great!
Date published: 2018-08-01
Rated 3 out of 5 by from マウス脳サンプルで使用 ウエスタン解析で使用。マウスIgGκ結合タンパク質と合わせて使用してみたが、少量で十分バンドが得られるため、お得感あり。今後も利用したい。
Date published: 2018-03-06
Rated 5 out of 5 by from Great IF staining! I tried this antibody as a sample to test before purchasing and it worked very well! This was tested at a 1:500 dilution in rat brain and worked exceptionally well.
Date published: 2018-02-09
  • y_2021, m_8, d_4, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_ko_KR, sid_271524, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT