MBD1 Antibody (A-5): sc-55473

MBD1 Antibody (A-5) is a mouse monoclonal IgG1 antibody that detects MBD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBD1, or methyl-CpG binding protein 1, plays a crucial role in the regulation of gene expression by binding to methylated DNA, particularly at methyl-CpG sites, which are often found in gene promoter regions. This binding is essential for mediating transcriptional repression, a process vital for normal cellular function and development. MBD1′s ability to interact with other proteins, such as histone deacetylases, further underscores MBD1′s importance in chromatin remodeling and gene silencing. Expression of MBD1 is notably high in somatic tissues, where MBD1 contributes to the maintenance of cellular identity and function by regulating gene transcription in response to DNA methylation changes. Anti-MBD1 antibody (A-5) is an invaluable tool for researchers studying epigenetic regulation and its implications in various biological processes and diseases.

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MBD1 Antibody (A-5) References:

1. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.  |  Reese, BE., et al. 2003. Mol Cell Biol. 23: 3226-36. PMID: 12697822
2. Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins.  |  Ichimura, T., et al. 2005. J Biol Chem. 280: 13928-35. PMID: 15691849
3. Effect of antisense MBD1 gene eukaryotic expression plasmid on expression of MBD1 gene in human biliary tract carcinoma cells.  |  Zuo, S., et al. 2005. J Huazhong Univ Sci Technolog Med Sci. 25: 658-61. PMID: 16696318
4. RNA interference of MBD1 in BxPC-3 human pancreatic cancer cells delivered by PLGA-poloxamer nanoparticles.  |  Luo, G., et al. 2009. Cancer Biol Ther. 8: 594-8. PMID: 19276660
5. Epigenetic regulation of miR-184 by MBD1 governs neural stem cell proliferation and differentiation.  |  Liu, C., et al. 2010. Cell Stem Cell. 6: 433-44. PMID: 20452318
6. Up-regulation of MBD1 promotes pancreatic cancer cell epithelial-mesenchymal transition and invasion by epigenetic down-regulation of E-cadherin.  |  Xu, J., et al. 2013. Curr Mol Med. 13: 387-400. PMID: 23331011
7. Silencing of MBD1 reverses pancreatic cancer therapy resistance through inhibition of DNA damage repair.  |  Xu, J., et al. 2013. Int J Oncol. 42: 2046-52. PMID: 23588667
8. Transcriptional repressor domain of MBD1 is intrinsically disordered and interacts with its binding partners in a selective manner.  |  Hameed, UF., et al. 2014. Sci Rep. 4: 4896. PMID: 24810720
9. Adult Neural Stem Cell Multipotency and Differentiation Are Directed by the Methyl-CpG-Binding Protein MBD1.  |  Lax, E. and Sapozhnikov, DM. 2017. J Neurosci. 37: 4228-4230. PMID: 28424299
10. Optochemical Control of TET Dioxygenases Enables Kinetic Insights into the Domain-Dependent Interplay of TET1 and MBD1 while Oxidizing and Reading 5-Methylcytosine.  |  Lin, TC., et al. 2022. ACS Chem Biol. 17: 1844-1852. PMID: 35709470

The preferred MBD1 antibody is MBD1 Antibody (B-5): sc-25261.