Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAS1 Double Nickase Plasmid (h): sc-401674-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAS1 Double Nickase Plasmid (h) and MAS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAS1 Antibody (G-1): sc-390453
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAS1 Double Nickase Plasmid (h) | sc-401674-NIC | 20 µg | $410.00 | |||
MAS1 Double Nickase Plasmid (h2) | sc-401674-NIC-2 | 20 µg | $410.00 |
MAS1 encodes the Mas1 proto-oncogene, a seven-transmembrane G protein-coupled receptor that functions as a key effector of the protective arm of the renin–angiotensin system through binding of angiotensin-(1–7). MAS1 signaling engages nitric oxide and prostaglandin pathways, modulates MAPK and PI3K/AKT cascades in a context-dependent manner, and influences vascular tone, inflammatory responses, and tissue remodeling. In human cells, MAS1 activity has been linked to regulation of endothelial function, fibrosis-associated signaling programs, and neurocardiovascular homeostasis. Dysregulated MAS1 expression or pathway balance has been investigated in cardiovascular and renal pathobiology, as well as in proliferative and invasive phenotypes reported in multiple tumor contexts.
MAS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.