Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lumican Double Nickase Plasmid (h): sc-402001-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lumican Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lumican Double Nickase Plasmid (h) and Lumican Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LUM. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lumican Antibody (B-9): sc-166871
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lumican Double Nickase Plasmid (h) | sc-402001-NIC | 20 µg | $410.00 | |||
Lumican Double Nickase Plasmid (h2) | sc-402001-NIC-2 | 20 µg | $410.00 |
LUM encodes lumican, a secreted small leucine-rich proteoglycan of the extracellular matrix that binds collagen fibrils and regulates fibrillogenesis, tissue hydration, and stromal architecture. Lumican modulates cell–matrix signaling and influences processes such as wound repair, cell migration, and mechanotransduction through interactions with integrins and growth factor availability within the matrix. In human tissues, LUM expression is prominent in cornea, skin, and connective stroma, where it contributes to collagen organization and transparency-related matrix properties. Dysregulated lumican levels and altered extracellular matrix remodeling have been associated with fibrotic remodeling, inflammatory microenvironments, and tumor–stroma interactions relevant to invasion and metastatic niche formation.
Lumican Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LUM locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LUM. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LUM function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LUM-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.