LOC100653515 CRISPR/Cas9 KO Plasmid (h): sc-418910
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC100653515 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOC100653515 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC100653515 CRISPR/Cas9 KO Plasmid (h) | sc-418910 | 20 µg | $397.00 |
Overview
CEP295NL (LOC100653515) is a human gene annotated as a CEP295-like locus and is expected to relate to centrosome-associated biology based on homology to CEP295 family members implicated in centriole biogenesis and centrosome maturation. Proteins in this functional space contribute to microtubule organization, spindle assembly, and faithful chromosome segregation during mitosis, thereby influencing cell-cycle progression and genome stability. Dysregulation of centrosome structure or copy number is frequently linked to aneuploidy and cellular stress phenotypes, providing a rationale for studying CEP295NL in proliferative contexts. Characterizing LOC100653515 can help clarify how centrosome-linked processes integrate with checkpoints and cytoskeletal remodeling pathways.
LOC100653515 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CEP295NL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CEP295NL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CEP295NL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOC100653515 protein expression.
This CRISPR knockout system enables efficient generation of CEP295NL-deficient cell models for investigation of LOC100653515 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CEP295NL exon(s) critical for LOC100653515 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CEP295NL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOC100653515 CRISPR/Cas9 KO Plasmid (h) and LOC100653515 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CEP295NL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOC100653515 HDR Plasmid (h) and LOC100653515 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CEP295NL homology arms to support homology-directed repair at defined CEP295NL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.