LOC100129480 Double Nickase Plasmid (h): sc-418668-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC100129480 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LOC100129480 Double Nickase Plasmid (h) and LOC100129480 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MKRN2OS. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC100129480 Double Nickase Plasmid (h) | sc-418668-NIC | 20 µg | $410.00 | |||
LOC100129480 Double Nickase Plasmid (h2) | sc-418668-NIC-2 | 20 µg | $410.00 |
MKRN2OS (human) is an antisense/overlapping transcript associated with the LOC100129480 locus and is expected to participate in locus-specific regulation of gene expression through RNA-mediated mechanisms such as transcriptional interference, chromatin modulation, or RNA stability control. The annotated protein product LOC100129480 remains poorly characterized, but its genomic context suggests potential links to ubiquitin-related signaling and post-transcriptional regulation that can influence cell-state decisions including proliferation, stress responses, and differentiation. Dysregulation of antisense transcription and noncoding RNA networks is frequently observed in cancer and other complex diseases, making MKRN2OS/LOC100129480 a useful target for mechanistic studies of gene regulation at bidirectional or overlapping loci. Functional interrogation can help clarify how this locus impacts RNA processing, proteostasis-associated pathways, and downstream transcriptional programs in relevant human cell models.
LOC100129480 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MKRN2OS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MKRN2OS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MKRN2OS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MKRN2OS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.