LLGL2 Antibody (A-4): sc-376857
- LLGL2 Antibody (A-4) is a mouse monoclonal IgG1 κ LLGL2 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 148-205 mapping within an internal region of LLGL2 of human origin
- LLGL2 Antibody (A-4) is recommended for detection of LLGL2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-LLGL2 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LLGL2 Antibody (A-4) for WB applications. These reagents are now offered in bundles with LLGL2 Antibody (A-4) (see ordering information below).
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LLGL2 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects LLGL2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-LLGL2 antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LLGL2, also known as lethal giant larvae homolog 2, is a crucial cortical cytoskeleton protein that plays a significant role in maintaining the organization and orientation of bipolar spindles during cell division, which is essential for proper mitotic processes. LLGL2 is part of a larger complex that includes LGN, protein kinase C i, and PAR-6β, and functions vitally for establishing epithelial cell polarity. In Drosophila melanogaster, LLGL2 is necessary for follicle cell differentiation, critical for proper ovarian development. In humans, LLGL2 regulates cell proliferation and has been associated with tumorigenesis, highlighting importance in cancer biology. Notably, LLGL2 phosphorylation during cell polarization may lead to segregation from PKC i and PAR-6β complex, influencing cellular dynamics and tight junction formation. LLGL2 is predominantly expressed in cytoplasm and localizes to perinuclear region of cells, underscoring roles in cellular architecture and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LLGL2 Antibody (A-4) References:
- Mammalian homolog of Drosophila tumor suppressor lethal (2) giant larvae interacts with basolateral exocytic machinery in Madin-Darby canine kidney cells. | Müsch, A., et al. 2002. Mol Biol Cell. 13: 158-68. PMID: 11809830
- Direct binding of Lgl2 to LGN during mitosis and its requirement for normal cell division. | Yasumi, M., et al. 2005. J Biol Chem. 280: 6761-5. PMID: 15632202
- aPKC, Crumbs3 and Lgl2 control apicobasal polarity in early vertebrate development. | Chalmers, AD., et al. 2005. Development. 132: 977-86. PMID: 15689379
- Expression of Hugl-1 is strongly reduced in malignant melanoma. | Kuphal, S., et al. 2006. Oncogene. 25: 103-10. PMID: 16170365
- Scribble associates with two polarity proteins, Lgl2 and Vangl2, via distinct molecular domains. | Kallay, LM., et al. 2006. J Cell Biochem. 99: 647-64. PMID: 16791850
- p32 is a novel mammalian Lgl binding protein that enhances the activity of protein kinase Czeta and regulates cell polarity. | Bialucha, CU., et al. 2007. J Cell Biol. 178: 575-81. PMID: 17682048
- Cloning and characterization of the promoter of Hugl-2, the human homologue of Drosophila lethal giant larvae (lgl) polarity gene. | Zimmermann, T., et al. 2008. Biochem Biophys Res Commun. 366: 1067-73. PMID: 18155665
- The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer. | Spaderna, S., et al. 2008. Cancer Res. 68: 537-44. PMID: 18199550
- Lethal(2)giant larvae is required in the follicle cells for formation of the initial AP asymmetry and the oocyte polarity during Drosophila oogenesis. | Li, Q., et al. 2008. Cell Res. 18: 372-84. PMID: 18268543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LLGL2 Antibody (A-4) | sc-376857 | 200 µg/ml | $322.00 | |||
LLGL2 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-529946 | 200 µg Ab; 10 µg BP | $361.00 | |||
LLGL2 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-523118 | 200 µg Ab, 40 µg BP | $361.00 | |||
LLGL2 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-543780 | 200 µg Ab; 20 µg BP | $361.00 | |||
LLGL2 Antibody (A-4) AC | sc-376857 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LLGL2 Antibody (A-4) HRP | sc-376857 HRP | 200 µg/ml | $322.00 | |||
LLGL2 Antibody (A-4) FITC | sc-376857 FITC | 200 µg/ml | $336.00 | |||
LLGL2 Antibody (A-4) PE | sc-376857 PE | 200 µg/ml | $349.00 | |||
LLGL2 Antibody (A-4) Alexa Fluor® 488 | sc-376857 AF488 | 200 µg/ml | $364.00 | |||
LLGL2 Antibody (A-4) Alexa Fluor® 546 | sc-376857 AF546 | 200 µg/ml | $364.00 | |||
LLGL2 Antibody (A-4) Alexa Fluor® 594 | sc-376857 AF594 | 200 µg/ml | $364.00 | |||
LLGL2 Antibody (A-4) Alexa Fluor® 647 | sc-376857 AF647 | 200 µg/ml | $364.00 | |||
LLGL2 Antibody (A-4) Alexa Fluor® 680 | sc-376857 AF680 | 200 µg/ml | $364.00 | |||
LLGL2 Antibody (A-4) Alexa Fluor® 790 | sc-376857 AF790 | 200 µg/ml | $364.00 |