LIF Antibody (E-10): sc-515931

LIF Antibody (E-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects LIF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-LIF antibody (E-10) is available as the non-conjugated form. LIF, also known as leukemia inhibitory factor, plays a crucial role in maintaining the pluripotency of embryonic stem (ES) cells by inhibiting their spontaneous differentiation, which is vital for stem cell research and potential therapeutic applications. This 202 amino acid secreted glycoprotein is produced by various cell types, including activated T lymphocytes, monocytes, mast cells, and neuronal cells, and is essential for promoting the survival and growth of axons in vitro. Additionally, LIF is implicated in immune tolerance at the maternal-fetal interface, suggesting its importance in reproductive biology. Beyond its role in stem cell maintenance, LIF may also influence fat and bone metabolism and is involved in the regulation of epithelial conversion during kidney development. The ability of LIF to modulate these processes highlights its significance in both developmental biology and potential clinical applications, making anti-LIF antibody (E-10) a valuable tool for researchers studying these complex biological systems.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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LIF Antibody (E-10) References:

1. Mesenchymal to epithelial conversion in rat metanephros is induced by LIF.  |  Barasch, J., et al. 1999. Cell. 99: 377-86. PMID: 10571180
2. Human embryonic stem cells.  |  Pera, MF., et al. 2000. J Cell Sci. 113 (Pt 1): 5-10. PMID: 10591620
3. p53 regulates maternal reproduction through LIF.  |  Hu, W., et al. 2007. Nature. 450: 721-4. PMID: 18046411
4. A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells.  |  Niwa, H., et al. 2009. Nature. 460: 118-22. PMID: 19571885
5. Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice.  |  Xu, J., et al. 2010. Cell Biol Int. 34: 791-7. PMID: 20392203
6. Leukemia inhibitory factor (LIF) enhances germ cell differentiation from primate embryonic stem cells.  |  Fukunaga, N., et al. 2010. Cell Reprogram. 12: 369-76. PMID: 20698776
7. Role of leukemia inhibitory factor in nasopharyngeal carcinogenesis.  |  Liu, SC. and Chang, YS. 2014. Mol Cell Oncol. 1: e29900. PMID: 27308310
8. Leukemia inhibitory factor (LIF) modulates the development of dendritic cells in a dual manner.  |  Yaftiyan, A., et al. 2019. Immunopharmacol Immunotoxicol. 41: 455-462. PMID: 31142168
9. Molecular cloning and expression of the human homologue of the murine gene encoding myeloid leukemia-inhibitory factor.  |  Gough, NM., et al. 1988. Proc Natl Acad Sci U S A. 85: 2623-7. PMID: 3128791
10. Plasma proteomics identifies leukemia inhibitory factor (LIF) as a novel predictive biomarker of immune-checkpoint blockade resistance.  |  Loriot, Y., et al. 2021. Ann Oncol. 32: 1381-1390. PMID: 34416362
11. Targeting leukemia inhibitory factor in pancreatic adenocarcinoma.  |  Wang, J., et al. 2023. Expert Opin Investig Drugs. 32: 387-399. PMID: 37092893
12. Leukemia inhibitory factor, a cytokine at the interface between neurobiology and immunology.  |  Patterson, PH. 1994. Proc Natl Acad Sci U S A. 91: 7833-5. PMID: 8058719

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LIF Antibody (E-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-LIF Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LIF Antibody (E-10) Specifications

Alternate Product Name: Leukemia Inhibitory Factor

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 81-102

Epitope Target: LIF

Epitope Species: human

Product Reactivity/Detection: LIF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for LIF Antibody (E-10): N/A