Lck 항체 (28) WB와 IF으로 mouse, rat와 human origin의 Lck을 검출할것을 권장합니다.

Lck 항체 (28): sc-135971

Lck 항체 (28) is rated 4.0 out of 5 by 4.
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데이터 시트

    • Lck 항체 28 는 마우스 monoclonal IgG1 이며 50 µg/0.5 ml으로 제공합니다.
    • human origin의 Lck에서 내부에 위치한 1-191 아미노산을 항원으로 사용하였습니다.
    • WB와 IF으로 mouse, rat와 human origin의 Lck을 검출할것을 권장합니다.; IP에는 권장하지않습니다.
    • See Lck (3A5): sc-433 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What is the recommended fixation method for immunofluorescence staining with Lck (28): sc-135971 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-18
Rated 4 out of 5 by from Strong bands and clean blots obtained using HuT 78 Strong bands and clean blots obtained using HuT 78, MOLT-4 and Ramos whole cell lysates. -SCBT QC
Date published: 2015-03-14
Rated 4 out of 5 by from Robust band in WB using Jurkat whole cell lysates Robust band in WB using Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-20
Rated 4 out of 5 by from Produced good immunofluorescence membrane Produced good immunofluorescence membrane staining in Jurkat cells. -SCBT QC
Date published: 2015-01-07
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