L-Caldesmon Antibody (F-1): sc-48427

L-Caldesmon Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects L-Caldesmon in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). L-Caldesmon, a low molecular weight isoform of Caldesmon, plays a crucial role in regulating actin filament dynamics, particularly in non-muscle tissues, where anti-L-Caldesmon antibody (F-1) reveals involvement in cell motility and shape maintenance. L-Caldesmon predominantly localizes in the cytoplasm and maintains structural integrity of the cytoskeleton, allowing cells to respond to mechanical stress and maintain shape. L-Caldesmon in non-muscle cells highlights importance in cellular functions beyond muscle contraction, as L-Caldesmon monoclonal antibody (F-1) shows interactions with other actin-binding proteins to modulate the actin cytoskeleton. L-Caldesmon undergoes post-translational modifications, such as phosphorylation, which can influence binding affinity and functional interactions, thereby impacting cellular signaling pathways. L-Caldesmon (F-1) antibody serves as an invaluable tool for researchers studying cytoskeleton dynamics and the role of actin-binding proteins in various physiological and pathological processes.

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L-Caldesmon Antibody (F-1) References:

1. Changes in Ca2+ signaling and contractile protein isoforms in smooth muscle cells from guinea pig ileum during culture.  |  Iijima, M., et al. 2001. J Smooth Muscle Res. 37: 53-66. PMID: 11592284
2. Phosphorylated l-caldesmon is involved in disassembly of actin stress fibers and postmitotic spreading.  |  Kordowska, J., et al. 2006. Exp Cell Res. 312: 95-110. PMID: 16289153
3. l-Caldesmon regulates proliferation and migration of vascular smooth muscle cells and inhibits neointimal formation after angioplasty.  |  Yokouchi, K., et al. 2006. Arterioscler Thromb Vasc Biol. 26: 2231-7. PMID: 16888241
4. Structural and functional relationships between h- and l-caldesmons.  |  Hayashi, K., et al. 1991. J Biol Chem. 266: 355-61. PMID: 1824698
5. Investigating copper-regulated protein expression in Menkes fibroblasts using antibody microarrays.  |  Du, T., et al. 2008. Proteomics. 8: 1819-31. PMID: 18384105
6. Histochemical localization of caldesmon in the CNS and ganglia of the mouse.  |  Köhler, CN. 2011. J Histochem Cytochem. 59: 504-17. PMID: 21411712
7. Histochemical localization of caldesmon isoforms in colon adenocarcinoma and lymph node metastases.  |  Köhler, C. 2011. Virchows Arch. 459: 81-9. PMID: 21626272
8. Effect of l-caldesmon on osteoclastogenesis in RANKL-induced RAW264.7 cells.  |  Liou, YM., et al. 2018. J Cell Physiol. 233: 6888-6901. PMID: 29377122
9. L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts.  |  Chan, CL., et al. 2019. J Biomed Sci. 26: 12. PMID: 30678675
10. Unraveling the mechanism in l-Caldesmon regulating the osteogenic differentiation of PDLSCs: An innovative perspective.  |  Li, Y., et al. 2024. Cell Signal. 118: 111147. PMID: 38513808

The preferred H-Caldesmon antibody is H-Caldesmon Antibody (h-CALD): sc-58703.