KCNMB4 항체 (B-6) WB, IP, IF 와ELISA으로 human origin의 KCNMB4을 검출할것을 권장합니다.

KCNMB4 항체 (B-6): sc-515712

KCNMB4 항체 (B-6) is rated 3.0 out of 5 by 1.
  • y_2021, m_6, d_15, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_ko_KR, sid_515712, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 132ms
  • REVIEWS, PRODUCT
loading
5
0
4
0
3
1
2
0
1
0

데이터 시트

    • 안티-KCNMB4 항체 B-6 는 마우스 monoclonal IgG2b (kappa light chain) KCNMB4 항체 이며 200 µg/ml으로 제공합니다.
    • 아미노 산152-168 within an extracellular domain of KCNMB4 of human origin 간의 피토프 매핑 에 특이적인다
    • KCNMB4 항체 (B-6)는 WB, IP, IF and ELISA으로 human유래의 KCNMB4 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-KCNMB4 항체 (B-6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • KCNMB4 (B-6): sc-515712무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KCNMB4 Antibody (B-6) for WB applications. These reagents are now offered in bundles with KCNMB4 Antibody (B-6) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using KCNMB4 (B-6): sc-515712?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2021, m_6, d_15, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_ko_KR, sid_515712, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 132ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from Risultato discreto Usato in Western blotting su cellule derivate da tumore al seno umano MCF7 e T47D, ha dato un segnale flebile intorno alle dimensioni attese secondo il data sheet; tuttavia, si sono sempre visualizzate 2 bande di peso molecolare piuttosto simile (forse isoforme della stessa proteina?). Inoltre risulta presente una banda, probabilmente aspecifica, di circa 35KDa. Globalmente, il risultato è da ritenersi sufficiente.
Date published: 2021-06-09
  • y_2021, m_6, d_15, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_ko_KR, sid_515712, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT