KANSL1 CRISPR/Cas9 KO Plasmid (h): sc-406711
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KANSL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KANSL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KANSL1 CRISPR/Cas9 KO Plasmid (h) | sc-406711 | 20 µg | $397.00 |
Overview
KANSL1 encodes a core component of the NSL (nonspecific lethal) histone acetyltransferase complex that cooperates with KAT8/MOF to acetylate histone H4 (notably H4K16ac) and regulate chromatin accessibility. Through this epigenetic activity, KANSL1 influences transcriptional programs linked to cell-cycle progression, DNA damage responses, and neurodevelopmental gene expression. Disruption of KANSL1 is strongly associated with Koolen–de Vries syndrome and has been implicated in broader neurodevelopmental phenotypes, making it a relevant target for mechanistic studies of chromatin-mediated regulation. Its functional connections to chromatin remodeling and transcriptional control support investigations into how altered histone acetylation states impact neuronal differentiation and gene network stability.
KANSL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KANSL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KANSL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KANSL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KANSL1 protein expression.
This CRISPR knockout system enables efficient generation of KANSL1-deficient cell models for investigation of KANSL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KANSL1 exon(s) critical for KANSL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KANSL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KANSL1 CRISPR/Cas9 KO Plasmid (h) and KANSL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KANSL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KANSL1 HDR Plasmid (h) and KANSL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KANSL1 homology arms to support homology-directed repair at defined KANSL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.