Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JAM-A Double Nickase Plasmid (h): sc-400687-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JAM-A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JAM-A Double Nickase Plasmid (h) and JAM-A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting F11R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JAM-A Antibody (J10.4): sc-53623
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JAM-A Double Nickase Plasmid (h) | sc-400687-NIC | 20 µg | $410.00 | |||
JAM-A Double Nickase Plasmid (h2) | sc-400687-NIC-2 | 20 µg | $410.00 |
F11R encodes junctional adhesion molecule A (JAM-A), an immunoglobulin superfamily receptor concentrated at tight junctions where it regulates epithelial and endothelial barrier integrity. JAM-A participates in cell–cell adhesion, polarity establishment, and contact-dependent signaling, and it modulates leukocyte transmigration through interactions with integrins and scaffolding proteins. Through coupling to junctional complexes and small GTPase pathways, JAM-A influences cytoskeletal organization and paracellular permeability. Dysregulated JAM-A expression or localization has been associated with inflammatory barrier dysfunction and altered cell migration phenotypes relevant to cancer biology and vascular pathology.
JAM-A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the F11R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within F11R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt F11R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of F11R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.