Date published: 2025-9-6

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Jagged1 항체 (E-12): sc-390177

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데이터 시트
  • Jagged1 항체 E-12 는 마우스 monoclonal IgG1 κ Jagged1 항체, 45간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 Jagged1에서 내부에 위치한 1110-1218 아미노산을 항원으로 사용하였습니다.
  • Jagged1 항체 (E-12)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Jagged1 를 감지하는 데에 추천한다.; 이외에 equine등 species와 반응할수 있습니다
  • Jagged1 항체(E-12)는 IP용으로 agarose에 결합된 형태로 제공됩니다. 그리고 IF, IHC(P) 및 FCM용으로 phycoerythrin 또는 FITC에 결합된 형태로 제공됩니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Jagged1 (E-12): sc-390177무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 Jagged1 항체 (E-12) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Jagged1 항체(E-12) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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Jagged1 항체(E-12)는 마우스, 쥐 및 인간 유래의 Jagged1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 Jagged1 항체(JAG1 항체, Protein jagged-1 또는 CD339 항체로도 지정됨)입니다. 재기드1 항체(E-12)는 비접합 항-재기드1 항체 형태와 아가로스, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-재기드1 항체 형태로 제공됩니다. LIN-12/Notch 계열의 막 통과 수용체는 세포의 운명 결정을 조절하여 발달에 중심적인 역할을 하는 것으로 알려져 있습니다. 노치의 리간드에는 재기드1, 재기드2 및 델타가 포함됩니다. 재기드는 막 단백질로, 근육 조절 및 구조 유전자의 발현을 억제하여 노치를 활성화하고 근아세포 분화를 방지할 수 있습니다. 포유류의 심혈관 발달과 조혈 과정 중 세포 운명 결정에 관여합니다. 재기드는 성인 및 태아 조직에서 발현되며 자궁 경부 편평 세포 암종에서 발현이 상향 조절됩니다. 가장 흔한 형태의 복합 선천성 심장병인 가족성 팔로트증은 재기드1 유전자의 돌연변이로 인해 발생합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Jagged1 항체 (E-12) 참고문헌:

  1. 알라길 증후군 환자의 JAGGED1 유전자에서 15개의 새로운 돌연변이.  |  Crosnier, C., et al. 2001. Hum Mutat. 17: 72-3. PMID: 11139247
  2. jagged1 유전자의 돌연변이로 인한 가족성 팔로트 증후군.  |  Eldadah, ZA., et al. 2001. Hum Mol Genet. 10: 163-9. PMID: 11152664
  3. 저산소증으로 유도된 lncHILAR는 miR-613/206/ 1-1-3p/Jagged-1/Notch/CXCR4 신호 경로의 ceRNA를 통해 신장암 전이를 촉진합니다.  |  Hu, G., et al. 2021. Mol Ther. 29: 2979-2994. PMID: 34058384
  4. 간세포 TLR4는 간세포 간 Jagged1/Notch 신호 전달을 촉발하여 NASH 유발 섬유화를 결정합니다.  |  Yu, J., et al. 2021. Sci Transl Med. 13: PMID: 34162749
  5. Nidogen-2는 혈관 평활근 세포의 수축 표현형을 유지하고 Jagged1-Notch3 신호 전달을 통해 신내막 형성을 방지합니다.  |  Mao, C., et al. 2021. Circulation. 144: 1244-1261. PMID: 34315224
  6. Jagged-1은 알레르기성 기도 질환에서 Th2 염증과 기억 세포 확장을 감소시킵니다.  |  Kimura, S., et al. 2023. Immunohorizons. 7: 168-176. PMID: 36729482
  7. 결함이 있는 jagged-1 신호는 GnRH 발달에 영향을 미치고 선천성 성선 자극 호르몬 결핍성 성선 기능 저하증에 기여합니다.  |  Cotellessa, L., et al. 2023. JCI Insight. 8: PMID: 36729644
  8. 지방 유래 중간엽 줄기세포는 Jagged-1/Notch-1/Hes 신호 전달 경로를 통해 조혈모세포의 증식을 촉진합니다.  |  Wang, H., et al. 2023. Stem Cells Int. 2023: 1068405. PMID: 38020206
  9. 재기드: Notch1을 활성화하는 포유류 리간드.  |  Lindsell, CE., et al. 1995. Cell. 80: 909-17. PMID: 7697721
  10. 소세포 폐암종과 신경내분비 종양 세포주에서 차별적으로 발현되는 포유류 상동 유전자로 추정되는 dlk.  |  Laborda, J., et al. 1993. J Biol Chem. 268: 3817-20. PMID: 8095043
  11. JAGGED2: 정단 외배엽 융기 및 상피-중간엽 상호작용 부위에서 발현되는 노치 리간드로 추정되는 단백질입니다.  |  Valsecchi, C., et al. 1997. Mech Dev. 69: 203-7. PMID: 9486542

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Jagged1 항체 (E-12)

sc-390177
200 µg/ml
$316.00

Jagged1 (E-12): m-IgG Fc BP-HRP 번들

sc-530084
200 µg Ab; 10 µg BP
$354.00

Jagged1 (E-12): m-IgGκ BP-HRP 번들

sc-523435
200 µg Ab, 40 µg BP
$354.00

Jagged1 (E-12): m-IgG1 BP-HRP 번들

sc-543860
200 µg Ab; 20 µg BP
$354.00

Jagged1 항체 (E-12) AC

sc-390177 AC
500 µg/ml, 25% agarose
$416.00

Jagged1 항체 (E-12) FITC

sc-390177 FITC
200 µg/ml
$330.00

Jagged1 항체 (E-12) PE

sc-390177 PE
200 µg/ml
$343.00

Jagged1 항체 (E-12) Alexa Fluor® 488

sc-390177 AF488
200 µg/ml
$357.00

Jagged1 항체 (E-12) Alexa Fluor® 546

sc-390177 AF546
200 µg/ml
$357.00

Jagged1 항체 (E-12) Alexa Fluor® 594

sc-390177 AF594
200 µg/ml
$357.00

Jagged1 항체 (E-12) Alexa Fluor® 647

sc-390177 AF647
200 µg/ml
$357.00

Jagged1 항체 (E-12) Alexa Fluor® 680

sc-390177 AF680
200 µg/ml
$357.00

Jagged1 항체 (E-12) Alexa Fluor® 790

sc-390177 AF790
200 µg/ml
$357.00

Have you tested this antibody for Western blot in MDA-MB-231 cells? If so, could you please share the transfer conditions, blocking method, and antibody dilutions you used?

Asked by: ssss
Thank you for your question. You can find our recommended western blot protocol under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2025-08-04

Hello, has anyone used this antibody, conjugated, for IHC on mouse samples?

Asked by: Ilinca
Thank you for your questions. We have only received publications for the unconjugated form for rat and human samples. If you happen to use the conjugated form for IHC you are covered under our 1 year warranty from date of purchase.
Answered by: BlakeJ
Date published: 2025-01-23

Can I use different positive controls than those mentioned in the datasheet (A-10 cell lysate, U-251-MG whole cell lysate, K-562 whole cell lysate)? For example: Jurkat of HEK293?

Asked by: mvisa
Thank you for your question. Yes, Jurkat and HEK293 can also be used for positive controls with this antibody .
Answered by: BlakeJ
Date published: 2023-09-19

Has anyone used the conjugated antibody with flow cytometry? What is the specificity like?

Asked by: jasper12
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-29

What is the preferred retrieval buffer for this antibody in IHC-P protocol?

Asked by: Souvik
Thank you for your question. We recommend using one of the following methods for antigen unmasking in formalin-fixed and paraffin embedded samples: 1 Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. 2 Pepsin: Incubate sections for 10–20 minutes in 0.1% Pepsin (sc-476554) in 0.01 N HCl at room temperature. Wash slides several times in deionized H2O. Aspirate excess liquid from slides. 3 Saponin: Incubate sections for 30 minutes in 0.05% Saponin (sc-280079) in deionized H2O at room temperature. Wash at least three times in PBS. Aspirate excess liquid from slides. You can find additional information in our recommended protocol in the 'Resources' section of our website.
Answered by: Tech Support Europe
Date published: 2019-09-05

What is the recommended storage temperature? How long is it stable at that temperature?

Asked by: science_man
Thank you for your question. We recommend storing this antibody at 4°C for long term storage. We guarantee it to be stable at this temperature for 1 year from the date of shipment.
Answered by: Technical Support
Date published: 2019-01-06

The expected size of JAG1 is 150kDa. I am looking in human neurons and see a very nice specific signal by ICC and WB, however, the band is around 60kDa (with nothing at higher molecular weights). Any thoughts on this? Looks great otherwise!

Asked by: Charlie777
Thank you for your question. The expected molecular weight of Jagged1 as detected by Jagged1 Antibody (E-12): sc-390177 is indeed 150 kDa. If you are detecting the protein at a different height please contact our technical service directly for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Does this antibody show any cross reactivity to Jagged-2?

Asked by: John25
Thank you for your question. Our mouse monoclonal Jagged1 Antibody (E-12): sc-390177 is specific for Jagged1 and does not cross-react with Jagged2. The antibody is raised against amino acids 1110-1218 of Jagged1 of human origin which does not share any significant sequence identity with Jagged2.
Answered by: Technical Support
Date published: 2017-02-16
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Rated 5 out of 5 by from JAG1 antibody worked well in immunofluorsenceMouse anti-human JAG1 antibody worked well in IF staining in human tissues.
Date published: 2023-10-09
Rated 4 out of 5 by from JAG1 worked very well by IHCJAG1 Ab worked well in histology in human tissues stained by DAB.
Date published: 2023-05-16
Rated 4 out of 5 by from buon anticorpo testato per WBgood antibody tested for western blot in human THP1 and LX2 total extract; good exposition time and clear band
Date published: 2022-11-21
Rated 4 out of 5 by from Good antibody.It's a very good antibody, not too strong. I used it for H9c2 cell lysate.
Date published: 2022-06-29
Rated 5 out of 5 by from Good antibody!1:200 diluted for wb of rat protein,the band is strong and special
Date published: 2018-03-21
Rated 4 out of 5 by from Jagged1 antibosy is good.I bought the antibody a month ago (free sample) and the WB result is good.
Date published: 2017-12-31
Rated 4 out of 5 by from good for western bloti received the sample antibody for this one and tried western blot on human skin tissue extracts. It shows a good band.
Date published: 2017-07-05
Rated 5 out of 5 by from very nice resultWB experiments are very successful, there can always be a clear band, WB effect is very good.
Date published: 2017-05-28
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Jagged1 항체 (E-12) is rated 4.7 out of 5 by 16.
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